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Published March 21, 2013 | Version 5020
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Incidence of Pathogenic Bacteria in Cakes and Tarts Displayed for Sale in Tripoli, Libya

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This study was conducted to investigate the incidence of pathogenic bacteria: Salmonella, Shigella, Escherichia coli O157 and Staphylococcus aureus in cakes and tarts collected from thirtyfive confectionery producing and selling premises located within Tripoli city, Libya. The results revealed an incidence of S. aureus with 94.4 and 48.0 %, E. coli O157 with 14.7 and 4.0 % and Salmonella sp. with 5.9 and 8.0 % in cakes and tarts samples respectively; while Shigella was not detected in all samples. In order to determine the source of these pathogenic bacteria, cotton swabs were taken from the hands of workers on the production line, the surfaces of preparation tables and cream whipping instruments. The results showed that the cotton swabs obtained from the hands of workers contained S. aureus and Salmonella sp. with an incidence of 42.9 and 2.9 %, the cotton swabs obtained from the surfaces of preparation tables 22.9 and 2.9 % and the cotton swabs obtained from the cream whipping instruments 14.3 and 0.0 % respectively; while E. coli O157 and Shigella sp. were not detected in all swabs. Additionally, other bacteria were isolated from the hands of workers and the Surfaces of producing equipments included: Aeromonas sp., Pseudomonas sp., E. coli, Klebsiella sp., Enterobacter sp., Citrobacter sp., Proteus sp., Serratia sp. and Acinetobacter sp. These results indicate that some of the cakes and tarts might pose threat to consumer's health. Meanwhile, occurrences of pathogenic bacteria on the hands of those who are working in production line and the surfaces of equipments reflect poor hygienic practices at most confectionery premises examined in this study. Thus, firm and continuous surveillance of these premises is needed to insure the consumer's health and safety.

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References

  • D.A.L. Seiler, "The microbiology of cake and its ingredients,". Food Trade Review. 48(6). pp. 339-344, 1978.
  • M. Kačániová, and L. Juhaniaková, "Microorganisms in Confectionery Products," Journal of Microbiology, Biotechnology and Food Sciences. 1(1). pp. 57-69, 2011.
  • J.F.R, Lues, and I. Van Tonder, "The occurrence of indicator bacteria on hands and aprons of food handlers in the delicatessen sections of a retail group,". Journal of food control. 18(4). pp. 326-332, 2007.
  • W. H. Andrews, and G. A. June, "Food sampling and preparation of sample homogenate," in: Food and drug administration:Bacteriology analytical manual. Revision, A. (Ed.). 8th ed. Association of Official Analytical Chemists International. USA, 1998, pp. 1-9.
  • W. H. Andrews, G. A. June, P. S. Sherrod, T. S. Hammack, and R. M. Amaguana, "Salmonella," in: Food and drug administration: Bacteriology analytical manual. Revision, A. (Ed.). 8th ed. Association of Official Analytical Chemists International. USA, 1998, pp. 1-9.
  • A. D. Hitchins, W. D. Watrins, S. R. Rippey, and L. Chandler, "Escherichia coli and coliform bacteria" in: Food and drug administration: Bacteriology analytical manual. Revision, A. (Ed.). 8th ed. Association of Official Analytical Chemists International. USA. 1998, pp. 1-9.
  • J. G. Cappuccino, and N. Sherman, Microbiology a laboratory manual. 5th.ed. Benjamin/ Cummings Science. New York, USA. 1998, pp. 151- 165, 179-184, 417-421.
  • R. W. Bennett, and G. A. Lancette, "Staphylococcus aureus," in: Food and drug administration: Bacteriology analytical manual. Revision, A. (Ed.). 8th ed. Association of Official Analytical Chemists International. USA, 1998, pp. 1-6.
  • M. Cheesbrough, Medical laboratory manual for tropical countries, vol II. The ford Press Ltd. UK. 1984, pp. 58-69, 225-227. [10] A. Viti, and G. Marchi, "Indexes of bacterial contamination in fresh confectionery produced in the district of Arezzo,". Industries Alimentary. 29(285). pp. 557-559, 1990. [11] S. Pla, P. Moreno, F. Fagoage, M. Rodriguez, M. Garcia, and A. Torregrosa, "Bacteriological control of cakes in the Alcoi health area,". Industries Alimentary. (270). pp. 51-53, 1996. [12] M. A. Estefo, and C. M, Savedra, "Bacteriological quality of Pastries manufactured in factories in the metropolitan region, Chile,". Journal of Food Protection, no. 275, pp. 137-141, 1996. [13] M. P. Doyle, T. Zhao, J. Meng, and S. Zhao, "Escherichia coli O157:H7," in: Food Microbiology Fundamentals and Frontiers American Society of Microbiology. Washington, USA. 1997, pp. 171-191. [14] FSAI Information Unit, "Guidelines for the interpretation of results of microbiological analysis of some ready-to-eat foods sampled at point of sale," Food Safety Authority of Ireland, 2001. [15] A. R. Eley, "Infective bacterial food poisoning," in: Microbial food poisoning. Eley. A. R. (Ed.). 1st.ed. Chapman & Hall. London, UK. 1992, pp. 15-55. [16] B. Siriken, O. Cadirci, G. Inat, and S. Pamuk, "Microbiological Examination of Meatball, Cream Cake and Turkish Delight (Lokum),". Journal of animal and veterinary advances. 8(10). pp. 2049 -2054, 2009. [17] G. M. Tebbutt, "A microbiological study of various food premises with an assessment of cleaning and disinfection practices,". Journal of Hygiene Companies. 92(2). pp. 365-375, 1984. [18] G. M. Tebbutt, and J. M. Southwell, "Comparative study of visual inspections and microbiological sampling in premises manufacturing and selling high-risk foods,". Epidemiology Infection. 103. pp. 475-486, 1989. [19] G. M. Tebbutt, "Development of standardized inspections in restaurants using visual assessments and microbiological sampling to quantify the risks,". Epidemiology Infection. 107. pp. 393-404, 1991. [20] J.G. Davis, "Microbiology of cream and dairy desserts," in: The Microbiology of Milk Products, Robinson, R.K. Vol II. Applied Science Publisher. London, UK. 1981, pp. 13-84.