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Ubiquitin-Proteasome Protocols pp 305–327Cite as

Aggresome Formation

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 301))

Summary

Bulk protein degradation in the cell is catalyzed by the ubiquitin-proteasome system (UPS). At the heart of the UPS is the proteasome, a large multisubunit tightly-regulated protease. The UPS performs key functions in protein quality control by monitoring and eliminating potentially toxic misfolded or damaged proteins. When the capacity of this protease system is exceeded, misfolded protein substrates aggregate and are assembled through an active and regulated process to form an aggresome. Aggresomes are dynamic structures, formed as a general response to an overload of improperly folded proteins. Assembly of aggresomes occurs at the centrosome, a perinuclear structure that also serves as a site for the recruitment and concentration of components of the UPS, including the proteasome, its regulators, and other proteins typically involved in protein quality control. Thus, in addition to other cellular activities, the centrosome may play a central role in protein quality control, sitting at the crossroads of protein folding, degradation, and aggregation.

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Acknowledgments

The authors wish to thank George DeMartino for helpful comments. This work was supported by research grants from the American Heart Association and the NIHNIDDK to P. J. T., the Cystic Fibrosis Foundation to M. J. C. and P. J. T., and the Haberecht Foundation to W. C. W.

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Authors and Affiliations

  1. Department of Physiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX

    Michael J. Corboy & Philip J. Thomas

  2. Reata Discovery, Inc., Dallas, TX

    W. Christian Wigley

Authors
  1. Michael J. Corboy
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  2. Philip J. Thomas
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  3. W. Christian Wigley
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Editor information

Editors and Affiliations

  1. Department od Medicine and the Carolina Cardiovascular Biology Center, University of North Carolina at Chapel Hill, Chapel Hill, NC

    Cam Patterson MD

  2. Department of Cell and Developmental Biology, University of North Carolona at Chapel Hill, Chapel Hill, NC

    Douglas M. Cyr PhD

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© 2005 Humana Press Inc.

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Corboy, M.J., Thomas, P.J., Wigley, W.C. (2005). Aggresome Formation. In: Patterson, C., Cyr, D.M. (eds) Ubiquitin-Proteasome Protocols. Methods in Molecular Biology™, vol 301. Humana Press. https://doi.org/10.1385/1-59259-895-1:305

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  • DOI: https://doi.org/10.1385/1-59259-895-1:305

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-252-0

  • Online ISBN: 978-1-59259-895-3

  • eBook Packages: Springer Protocols

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