Abstract
The reagent 1-dimethylaminonaphthalene-5-sulfonyl chloride (dansyl chloride, DNS-Cl) reacts with the free amino groups of pep-tides and proteins as shown in Fig. 1. Total acid hydrolysis of the substituted peptide or protein yields a mixture of free amino acids plus the dansyl derivative of the N-terminal amino acid, the bond between the dansyl group and the N-terminal amino acid being resistant to acid hydrolysis. The dansyl amino acid is fluorescent under UV light and is identified by thin-layer chromatography on polyamide sheets. This is an extremely sensitive method for identifying amino acids and in particular has found considerable use in peptide sequence determination when used in conjunction with the Edman degradation (see Chapter 36). The dansyl technique was originally introduced by Gray and Hartley (1), and was developed essentially for use with peptides. However, the method can also be applied to proteins (see Note 1).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Gray, W. R. and Hartley, B. S. (1963) A fluorescent end group reagent for peptides and proteins. Biochem. J. 89, 59P.
Sutton, M. R. and Bradshaw, R. A. (1978) Identification of dansyl dipeptides. Anal. Biochem. 88, 344–346.
Gray, W. R. (1967) in Methods in Enzymology, vol. XI (Hirs, C. H. W., ed.), p. 149. Academic, New York.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1994 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Walker, J.M. (1994). The Dansyl Method for Identifying N-Terminal Amino Acids. In: Walker, J.M. (eds) Basic Protein and Peptide Protocols. Methods in Molecular Biology™, vol 32. Humana Press. https://doi.org/10.1385/0-89603-268-X:321
Download citation
DOI: https://doi.org/10.1385/0-89603-268-X:321
Publisher Name: Humana Press
Print ISBN: 978-0-89603-268-2
Online ISBN: 978-1-59259-519-8
eBook Packages: Springer Protocols