Abstract
Plant biomass is the main type of organic material on Earth. The efficiency of biocatalytic conversion of plant raw materials determines the cost of their biotechnological processing to produce commercially valuable products such as organic alcohols and acids, carbohydrates, and hydrocarbons. New recombinant Penicillium canescens strains that produce not only their own enzyme complex but also heterologous cellulases (i.e., mutant and wild-type cellobiohydrolase I (CBH I) and endoglucanase II (EG II) of P. verruculosum) are constructed. Enzymatic agents (EAs) prepared on the basis of recombinant strains of P. canescens are found to be more active in the hydrolysis of crushed aspen wood. Yields of glucose and reducing sugars are observed 24–72 h after hydrolysis with EAs prepared in recombinant strains to be from 48 to 52 and 60 to 64%, respectively, higher than those for hydrolysis with EAs prepared in the initial recipient strain. The presence of N45A and N194A site-specific mutations introduced to reduce surface glycosilation thus results in a substantial increase in the yields of desired CBH I and EG II.
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References
Bungay, H.R., Energy: the Biomass Options, New York Wiley, 1981.
Bioprocessing of Renewable Resources to Commodity Bioproducts, Bisaria, V.S. and Kondo, A., Eds., New York, Wiley, 2014.
Sticklen, M., Curr. Opin. Biotechnol., 2006, vol. 17, no. 3, pp. 315–319.
From the Sugar Platform to biofuels and biochemicals. Final report for the European Commission Directorate- General Energy no. ENER/C2/423-2012/SI2.673791. https://ec.europa.eu/energy/sites/ener/files/documents/EC%20Sugar%20Platform%20final%20report.pdf. Cited January 14, 2017.
Kumar, R., Singh, S., and Singh, O.V., J. Ind. Microbiol. Biotechnol., 2008, vol. 35, no. 5, pp. 377–391.
Chekushina, A.V., Dotsenko, G.S., and Sinitsyn, A.P., Catal. Ind., 2013, vol. 5, no. 1, pp. 98–104. doi 10.1134/S2070050413010042
Martins, L.F., Kolling, D., Camassola, M., Dillion, A.J.P., and Ramos, L.P., Bioresour. Technol., 2008, vol. 99, no. 5, pp. 1417–1424.
Ikeda, Y., Hayashi, H., Okuda, N., and Park, E.Y., Biotechnol. Prog., 2007, vol. 23, no. 2, pp. 333–338.
Biotechnology for Agro-Industrial Residues Utilization, Nigam, P. and Pandley, A., Eds., New York, Springer, 2009.
Illanes, A., Cauerhff, A., Wilson, L., and Castro, G.R., Bioresour. Technol., 2012, vol. 115, pp. 48–57.
Gusakov, A.V. and Sinitsyn, A.P., Biofuels, 2012, vol. 3, no. 4, pp. 463–477.
Dotsenko, A.S., Gusakov, A.V., Volkov, P.V., Rozhkova, A.M., and Sinitsyn, A.P., Biotechnol. Bioeng., 2016, vol. 113, no. 2, pp. 283–291.
Dotsenko, A.S., Gusakov, A.V., Rozhkova, A.M., Sinitsyna, O.A., Nemashkalov, V.A., and Sinitsyn, A.P., Protein Eng., Des. Sel., 2016, vol. 29, no. 11, pp. 495–502. doi 10.1093/protein/gzw030
Aslanidis, C. and De Jong, P.J., Nucleic Acids Res., 1990, vol. 18, no. 20, pp. 6069–6074.
Sinitsyn, A.P. and Rozhkova, A.M., in Microorganisms in Biorefineries, Kamm, B., Ed., Berlin: Springer, 2015, pp. 1–19.
Aleksenko, A.Y., Makarova, N.A., Nikolaev, I.V., and Clutterbuck, A.J., Curr. Genet., 1995, vol. 28, no. 5, pp. 474–477.
Nelson, N.A., J. Biol. Chem., 1944, vol. 153, no. 2, pp. 375–380.
Somogyi, M., J. Biol. Chem., 1952, vol. 195, no. 1, pp. 19–23.
Peterson, G.L., Anal. Biochem., 1979, vol. 100, no. 2, pp. 201–220.
Morozova, V.V., Gusakov, A.V., Andrianov, R.M., Pravilnikov, A.G., Osipov, D.O., and Sinitsyn, A.P., Biotechnol. J., 2010, vol. 5, no. 8, pp. 871–880.
Chekushina, A.V., Dotsenko, G.S., Kondratieva, E.G., and Sinitsyn, A.P., Biotechnol. Russ., 2013, no. 3, pp. 58–68.
Chekushina, A.V., Dotsenko, G.S., Kondratieva, E.G., and Sinitsyn, A.P., Biotechnol. Russ., 2013, no. 3, pp. 69–80.
Smirnova, I.A., Sereda, A.S., Kostyleva, E.V., Tsurikova, N.V., Bushina, E.V., Rozhkova, A.M., and Sinitsyn, A.P., Appl. Biochem. Microbiol., 2015, vol. 51, no. 6, pp. 660–666.
Clarke, A.J., Biodegradation of Cellulose. Enzymology and Biotechnology, Lancaster, PA Technomic, 1997.
Teeri, T.T., Trends Biotechnol., 1997, vol. 15, no. 5, pp. 160–167.
Woods, T.M., McCrae, S.I., and Bhat, K.M., Biochem. J., 1989, vol. 260, no. 1, pp. 37–43.
Polizeli, M.L.T.M., Rizzatti, A.C.S., Monti, R., Terenzi, H.F., Jorge, J.A., and Amorim, D.S., Appl. Microbiol. Biotechnol., 2005, vol. 67, no. 5, pp. 577–591.
Berlin, A., Maximenko, V., Gilkes, N., and Saddler, J., Biotechnol. Bioeng., 2007, vol. 97, no. 2, pp. 287–296.
Wieczorek, A.S., Biot-Pelletier, D., and Martin, V.J.J., in Cellulose—Biomass Conversion, van De Ven, T. and Kadla, J., Eds., Rijeka, Croatia InTech}, 2013, ch. 5, pp. 101–130. http://www.intechopen.com/books/cellulose-biomass-conversion/recombinant-cellulase-andcellulosome- systems. Cited 14 January}, 2017. doi 10.5772/54225
Proskurina, O.V., Korotkova, G.G., Rozhkova, A.M., Matys, V.Yu., Koshelev, A.V., Okunev, O.N., Nemashkalov, V.A., Sinitsyna, O.A., Revin, V.V., and Sinitsyn, A.P., Catal. Ind., 2014, vol. 6, no. 1, pp. 72–78. doi 10.1134/S2070050414010085
National Renewable Energy Laboratory Website. www.nrel.gov/. Cited 14 January, 2017.
Li, D.-C., Li, A.-N., and Papageorgiou, A.C., Enzyme Res., 2011. https://www.ncbi.nlm.nih.gov/pmc/articles/ PMC3226318. Cited 14 January, 2017. doi 10.4061/ 2011/308730
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Original Russian Text © A.S. Dotsenko, A.M. Rozhkova, A.V. Gusakov, A.P. Sinitsyn, 2016, published in Kataliz v Promyshlennosti.
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Dotsenko, A.S., Rozhkova, A.M., Gusakov, A.V. et al. Improving the efficiency of the bioconversion of plant raw materials with mutant cellulases of Penicillium verruculosum . Catal. Ind. 9, 71–76 (2017). https://doi.org/10.1134/S2070050417010044
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DOI: https://doi.org/10.1134/S2070050417010044