Protocol

Injection of Chemicals into Pupae of the African Butterfly Bicyclus anynana

  1. Bas J. Zwaan
  1. Institute of Biology, Leiden University, 2300 RA Leiden, The Netherlands
  1. 1Corresponding author (p.m.brakefield{at}biology.leidenuniv.nl)
This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.

INTRODUCTION

This protocol describes a procedure for the injection of chemicals into pupae of the African butterfly Bicyclus anynana. A number of target traits can be monitored to determine the effect of a particular chemical on the pupae.

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