Hemolymph Extraction from Various Developmental Stages of the African Butterfly Bicyclus anynana

Paul M. Brakefield; Patrícia Beldade; Bas J. Zwaan

doi:10.1101/pdb.prot5214

Protocol

Hemolymph Extraction from Various Developmental Stages of the African Butterfly Bicyclus anynana

Institute of Biology, Leiden University, 2300 RA Leiden, The Netherlands

↵¹Corresponding author (p.m.brakefield{at}biology.leidenuniv.nl)

This article is also available in Emerging Model Organisms: A Laboratory Manual, Vol. 1. CSHL Press, Cold Spring Harbor, NY, USA, 2009.

INTRODUCTION

This protocol describes procedures for the extraction of hemolymph from larvae, pupae, and adults of the African butterfly Bicyclus anynana. The hemolymph can subsequently be used for various purposes; the method for processing the hemolymph depends on the question under investigation.