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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 57, Issue 2

gen. nov., sp. nov., the square haloarchaeon of Walsby, isolated from saltern crystallizers in Australia and Spain Free

Abstract

Strains C23 and HBSQ001 were isolated from solar salterns and are novel square-shaped, aerobic, extremely halophilic members of the domain and family . Cells stained Gram-negative and grew optimally in media containing 18 % salts at around neutral pH. Mg is not required. The DNA G+C content of both isolates was 46.9 mol% and DNA–DNA cross-hybridization showed a relatedness of 80 %. Their 16S rRNA gene sequences showed only 2 nucleotide differences (99.9 % identity) and phylogenetic tree reconstructions with other recognized members of the indicated that they formed a distinct clade, with the closest relative being PR 3 (91.2 % sequence identity). The major polar glycolipid of both isolates was the sulfated diglycosyl diether lipid S-DGD-1. Electron cryomicrosopy of whole cells revealed similar internal structures, such as gas vesicles and polyhydroxyalkanoate granules, but the cell wall of isolate HBSQ001 displayed a more complex S-layer compared with that of isolate C23. The phenotypic characterization and phylogenetic data support the placement of isolates C23 and HBSQ001 in a novel species in a new genus within the , for which we propose the name gen. nov., sp. nov. The type strain of is C23 (=JCM 12705=DSM 16854).

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Keyword(s): PHA, polyhydroxyalkanoate and SHOW, square haloarchaea of Walsby
SGM
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2007-02-01
2024-04-20
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Haloquadratum walsbyi gen. nov., sp. nov., the square haloarchaeon of Walsby, isolated from saltern crystallizers in Australia and Spain
Int J Syst Evol Microbiol 57, 387 (2007); https://doi.org/10.1099/ijs.0.64690-0
/content/journal/ijsem/10.1099/ijs.0.64690-0
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