Issue 4, 2022
From the journal:

Materials Chemistry Frontiers

One-step light-up metabolic probes for in situ discrimination and killing of intracellular bacteria†

Eric Y. Yu,a   Michelle M. S. Lee,a   Joe H. C. Chau,a   Kristy W. K. Lam,a   Hojeong Park, ORCID logo a   Ryan T. K. Kwok,*ab   Jacky W. Y. Lam,ab   Yuanyuan Li*bc  and  Ben Zhong Tang ORCID logo *abde  

* Corresponding authors

a Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, State Key Laboratory of Neuroscience, and Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
E-mail: chryan@ust.hk, tangbenz@ust.hk

b HKUST-Shenzhen Research Institute, No. 9 Yuexing 1st Rd, South Area Hi-Tech Park, Nanshan, Shenzhen 518057, China
E-mail: li_yuanyuan@jlu.edu.cn

c College of Veterinary Medicine, Jilin University, Changchun 130062, China

d Shenzhen Institute of Aggregate Science and Technology, School of Science and Engineering, The Chinese University of Hong Kong, Shenzhen, 2001 Longxiang Boulevard, Longgang District, Shenzhen City, Guangdong 518172, China

e State Key Laboratory of Luminescent Materials and Devices, and Center for Aggregation-Induced Emission (Guangzhou International Campus), South China University of Technology, Guangzhou 510640, China

Abstract

Bacterial infections and the rise of antibiotic-resistance pose significant threats to public health, which calls for a profound change in diagnosis and treatment. Considering this, we develop two multi-functional light-up metabolic probes, namely TPEPy-Ala and TPAPy-Kdo, based on aggregation-induced emission (AIE) luminogens with photosensitizing properties. These AIE light-up probes can not only metabolically label both Gram-positive and Gram-negative bacteria with a high signal-to-noise contrast but also visualize the bacteria internalized in cells. Furthermore, these photosensitizing probes could in situ generate reactive oxygen species (ROS) upon light irradiation to effectively kill the intracellular bacteria. More importantly, both TPEPy-Ala and TPAPy-Kdo only exert minimal side effects on the host macrophages. This work presents a one-step light-up metabolic approach to discriminate and kill bacteria simultaneously with high specificity by utilizing their respective metabolic pathways.

Graphical abstract: One-step light-up metabolic probes for in situ discrimination and killing of intracellular bacteria

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Article information

DOI
https://doi.org/10.1039/D1QM01495A
Article type
Research Article
Submitted
09 Nov 2021
Accepted
27 Dec 2021
First published
14 Jan 2022

Mater. Chem. Front., 2022,6, 450-458

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One-step light-up metabolic probes for in situ discrimination and killing of intracellular bacteria

E. Y. Yu, M. M. S. Lee, J. H. C. Chau, K. W. K. Lam, H. Park, R. T. K. Kwok, J. W. Y. Lam, Y. Li and B. Z. Tang, Mater. Chem. Front., 2022, 6, 450 DOI: 10.1039/D1QM01495A

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