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p15PAF, a novel PCNA associated factor with increased expression in tumor tissues

Oncogene volume 20, pages 484–489 (2001)Cite this article

Abstract

Proliferating cell nuclear antigen (PCNA) is an essential protein in both DNA replication and DNA damage repair. A novel 15 kD protein, p15PAF, was identified as a PCNA-associated factor in a yeast two-hybrid screen using PCNA as the bait. p15PAF is localized primarily in the nucleus. p15PAF shares the conserved PCNA binding motif with several other PCNA binding proteins including CDK inhibitor p21. Overexpression of p15PAF competes with p21-PCNA binding. Mutation of this motif in p15PAF abolished its PCNA-binding activity. Notably, p15PAF expression in several types of tumor tissues was significantly increased, especially in esophageal tumors. Like PCNA, p15PAF may possess prognostic significance in a broad array of human cancers.

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Acknowledgements

We thank Jim Lorens and Susan Demo, for their help during experimental design. We would also like to thank Karla Blonsky for her critical reading of the manuscript.

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Authors and Affiliations

  1. Rigel Pharmaceuticals, Inc., 240 East Grand Avenue, South San Francisco, CA 94080, California, USA

    Peiwen Yu, Betty Huang, Mary Shen, Clorinda Lau, Eva Chan, Donald G Payan & Ying Luo

  2. Biochemistry and Biophysics Department, 22-012 Lineberger Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7295, North Carolina, USA

    Jennifer Michel & Yue Xiong

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  1. Peiwen Yu
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Yu, P., Huang, B., Shen, M. et al. p15PAF, a novel PCNA associated factor with increased expression in tumor tissues. Oncogene 20, 484–489 (2001). https://doi.org/10.1038/sj.onc.1204113

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