Abstract
The Rho small G protein family, consisting of the Rho, Rac, and Cdc42 subfamilies, regulates various actin cytoskeleton-dependent cell functions. The Rho subfamily members regulate ERM (ezrin, radixin and moesin)-dependent association of the actin cytoskeleton with the plasma membrane. Moreover, the N-terminal regions of ERM interact with Rho GDI, an inhibitory regulator of all the Rho family members, and reduce its inhibitory action, finally initiating the activation of the Rho family members. We show here that the N-terminal region of radixin furthermore interacts with Dbl, a stimulatory GDP/GTP exchange protein of the Rho family members. This interaction does not affect the Dbl activity to stimulate the GDP/GTP exchange reaction of RhoA, a member of the Rho subfamily. Dbl does not interact with radixin which is precomplexed with Rho GDI, and Rho GDI displaces Dbl from radixin. Thus, radixin plays an important role in activation of the Rho family members by recruiting their positive and negative regulators.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 50 print issues and online access
$259.00 per year
only $5.18 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Takahashi, K., Sasaki, T., Mammoto, A. et al. Interaction of radixin with Rho small G protein GDP/GTP exchange protein Dbl. Oncogene 16, 3279–3284 (1998). https://doi.org/10.1038/sj.onc.1201874
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/sj.onc.1201874
Keywords
This article is cited by
-
The 'invisible hand': regulation of RHO GTPases by RHOGDIs
Nature Reviews Molecular Cell Biology (2011)
-
Dystroglycan versatility in cell adhesion: a tale of multiple motifs
Cell Communication and Signaling (2010)
-
Organizing the cell cortex: the role of ERM proteins
Nature Reviews Molecular Cell Biology (2010)
-
Phosphorylation-independent membrane relocalization of ezrin following association with Dbl in vivo
Oncogene (2004)
-
Moesin functions antagonistically to the Rho pathway to maintain epithelial integrity
Nature (2003)