Abstract
THE mechanism of transformation of the overtly similar cells of the neural plate into the numerous and diverse cell types of the mature vertebrate central nervous system (CNS)1 can better be understood by studying the clonal development of isolated CNS precursor cells. Here I describe a culture system in which blast cells (cells capable of division) isolated from embryonic day 13.5–14.5 rat forebrain can divide and differentiate into a variety of clonal types. Most clones contain only neurons or glia; 22% contain both neurons and non-neuronal cells. For the division of blast cells, live conditioning cells need to be present indicating that environmental signals influence proliferation. Heterogeneous clones develop in homogeneous culture conditions, so factors intrinsic to the blast cells are probably important in determining the number and type of clonal progeny.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Jacobson, M. Developmental Neurobiology 2nd edn (Plenum, New York & London, 1978).
Bayer, S. A. J. comp. Neurol. 183, 89–106 (1979).
Bayer, S. A. J. comp. Neurol. 183, 107–120 (1979).
Bignami, A., Eng, L. F., Dahl, D. & Uyeda, C. T. Brain Res. 43, 429–435 (1972).
Abney, E. R., Bartlett, P. P. & Raff, M. C. Devl Biol. 83, 301–310 (1981).
Turner, D. L. & Cepko, C. L. Nature 328, 131–136 (1987).
Wetts, R. & Fraser, S. E. Science 239, 1142–1145 (1988).
Holt, C. E., Bertsch, T. W., Ellis, H. M. & Harris, W. A. Neuron 1, 15–26 (1988).
Walsh, C. & Cepko, C. L. Science 241, 1342–1345 (1988).
De Vitry, F. et al. Proc. natn. Acad. Sci. U.S.A. 77, 4165–4169 (1980).
Frederiksen, K., Jat, P. S., Valtz, N., Levy, D. & McKay, R. Neuron 1, 439–448 (1988).
Lathja, L. G. Differentiation 14, 23–24 (1979).
Temple, S. & Raff, M. C. Nature 313, 223–225 (1985).
Kawamoto, J. C. & Barrett, J. N. Brain Res. 384, 84–93 (1986).
Kaufman, L. M. & Barrett, J. N. Science 220, 1394–1396 (1983).
Pruss, R. M. Nature 280, 688–690 (1979).
Johnson, G. D. et al. J. immunol. Meth. 55, 231–242 (1982).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Temple, S. Division and differentiation of isolated CNS blast cells in microculture. Nature 340, 471–473 (1989). https://doi.org/10.1038/340471a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/340471a0
This article is cited by
-
The role of m6A modification in the biological functions and diseases
Signal Transduction and Targeted Therapy (2021)
-
Analysis of clonogenic growth in vitro
Nature Protocols (2021)
-
Deciphering neural heterogeneity through cell lineage tracing
Cellular and Molecular Life Sciences (2021)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.