Elsevier

Genomics

Volume 87, Issue 1, January 2006, Pages 181-190
Genomics

Method
Utilization of a zebra finch BAC library to determine the structure of an avian androgen receptor genomic region

https://doi.org/10.1016/j.ygeno.2005.09.005Get rights and content
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Abstract

The zebra finch (Taeniopygia guttata) is an important model organism for studying behavior, neuroscience, avian biology, and evolution. To support the study of its genome, we constructed a BAC library (TG__Ba) using DNA from livers of females. The BAC library consists of 147,456 clones with 98% containing inserts of an average size of 134 kb and represents 15.5 haploid genome equivalents. By sequencing a whole BAC, a full-length androgen receptor open reading frame was identified, the first in an avian species. Comparison of BAC end sequences and the whole BAC sequence with the chicken genome draft sequence showed a high degree of conserved synteny between the zebra finch and the chicken genome.

Keywords

Androgen receptor
BAC library
Bird
Genomics
Zebra finch

Cited by (0)

Sequence data from this article have been deposited with the GenBank Data Library under Accession Nos. CW991863 through CW991926 and CZ167565 to CZ167566 for the BAC end sequences, AC153487 for the BAC 319A15 sequence, AY847476 for the AR 5′RACE product, and BK005685 for our predicted zebra finch AR cDNA (pzfAR).

1

Current address: Biotechnology Institute, University of Maryland, College Park, MD 20742, USA.