Case reportEvidence of circulation of Laguna Negra-like hantavirus in the Central West of Brazil: Case report
Section snippets
Background
Hantaviruses are serologically related members of the family Bunyaviridae that occur worldwide in association with rodents and insectivore carriers. These viruses have been linked with two distinct diseases: hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS).1
In Brazil, a surveillance program was implemented in 1993 to monitor HPS cases.2 Afterwards, evidence for hantavirus infection was described in various regions. In order to identify the hantavirus strain
Objectives
The objective of this study is identifying the hantavirus strain related to a fatal HPS case in Mato Grosso state, Brazil and determined its phylogenetic relationship with circulating South American hantaviruses.
Study design
Serum and clot samples from a patient with suggestive symptoms of hantavirus infection were tested using specific IgM and IgG antibody assays (ELISA) and RT-PCR for viral RNA detection. Partial viral genomic S segment was sequenced and genotyped by phylogenetic reconstruction. In addition, histopathological and immunohistochemical studies were performed in tissues sections of lung and liver aiming to characterize the main target sites during hantavirus infection.
Results
The patient was a 19-year-old man farm worker, previously healthy, who lived in Campo Novo do Parecis, in the west of Mato Grosso (13°40′31″S 57°53′31″W). He was admitted to hospital reporting 3 days of flu-like symptoms, and after hospitalization, he developed malaise, high fever, myalgia, headache, dry cough, tiredness, dispnea, back pain, nausea, vomits, abdominal pain and diarrhea. The initial treatment was intravenous administration of one liter of normal saline solution and symptomatic
Discussion
In Brazil, despite the increasing training of medical personnel to recognize the clinical symptoms of HPS, mortality rate is still high (∼40%). The demarcation of hantavirus transmission areas and the characterization of circulating virus should contribute to better direct control measures. To identify its causative agent, we studied a fatal HPS case from the state of Mato Grosso, in the central region of Brazil. This area is of special interest because it presents a growing number of
Conflict of interest
The authors have declared that no competing interests exist.
Acknowledgments
We are indebted with Gisélia Rubio from Departamento de Vigilância, SESA-PR and Irene Skraba from LACEN-PR. The authors thank CNPq, CNPq/Prosul, Fiocruz, Fundação Araucária, CYTED/RIVE and Fundo Paraná for financial support. LB, FGH, SC, GACA, JB and CNDS are CNPq fellowship recipients.
References (23)
- et al.
Hantavirus infection in Brazil: development and evaluation of an enzyme immunoassay and immunoblotting based on N recombinant protein
Diagn Microbiol Infect Dis
(2007) - et al.
Laguna Negra virus associated with HPS in western Paraguay and Bolivia
Virology
(1997) - et al.
Hantaviruses and hantavirus pulmonary syndrome
Clin Infect Dis
(1994) - Coordenação de Controle de Vetores e Antropozoonoses SdVeSMdSB. Dados do Programa de Controle da Hantavirose no Brasil...
- et al.
Genetic investigation of novel hantaviruses causing fatal HPS in Brazil
J Med Virol
(1999) - et al.
Identifying rodent hantavirus reservoirs, Brazil
Emerg Infect Dis
(2004) - et al.
Hantaviruses in São Paulo State, Brazil
Emerg Infect Dis
(2003) - et al.
Newly recognized hantaviruses associated with hantavirus pulmonary syndrome in northern Brazil: partial genetic characterization of viruses and serologic implication of likely reservoirs
Vector Borne Zoonotic Dis
(2005) - et al.
Expression of a hantavirus N protein and its efficacy as antigen in immune assays
Braz J Med Biol Res
(2008) - et al.
Associação dos casos de síndrome cardiopulmonar por hantavírus na área de influência da BR 324 com o vírus Laguna Negra
Identificação do vírus Castelo dos Sonhos em roedores Oligoryzomys moojeni em Campo Novo do Parecis
Cited by (22)
Orthohantavirus pulmonary syndrome in Santa Cruz and Tarija, Bolivia, 2018
2020, International Journal of Infectious DiseasesCitation Excerpt :All orthohantaviruses known to cause human disease are associated with rodent hosts (Montgomery et al., 2012). To date, at least 37 main species or genotypes of orthohantavirus have been identified as being in circulation in Bolivia, including the following: Rio Mamore virus (RIOMV) (Bharadwaj et al., 1997; Hjelle et al., 1996) (also reported in Peru and the French Guiana (Powers et al., 1999; Richter et al., 2010)), Laguna Negra virus (LANV) or LANV-like viruses (also reported in Paraguay, Brazil, and Argentina) (Johnson et al., 1997; Levis et al., 2004; Raboni et al., 2009), Bermejo virus (BMJV), reported explicitly in Bolivia (Montgomery et al., 2012; Montoya-Ruiz et al., 2014), and Andes virus (ANDV) strains, including new ones such as Tunari virus (TUNV) (Cruz et al., 2012). Bermejo is a city and municipality in the extreme south of Tarija department in Bolivia, where two cases of orthohantavirus pulmonary or cardiopulmonary syndrome (OHCPS) occurred in May and July 2000.
Label-free electrochemical immunosensor for quick detection of anti-hantavirus antibody
2019, Journal of Electroanalytical ChemistryCitation Excerpt :Hantavirus Araucaria nucleoprotein (HNp) 1.16 mg mL−1, monoclonal anti-hantavirus Araucaria antibody IgG2b (Ab), 0.4 mg mL−1 and control antibody 0.16 mg mL−1 were obtained from the Laboratory of Molecular Virology at Carlos Chagas Institute/Fiocruz PR – Brazil. All the biomolecules used were provided by the Molecular Virology Laboratory at Carlos Chagas Institute/Fiocruz PR – Brazil, and the methods of production and purification of the Hantavirus nucleoprotein and monoclonal antibody are described by Raboni et al. [4,23]. Control antibody has the same route of production and common structure to the Ab [23].
Detection of different South American hantaviruses
2015, Virus ResearchCitation Excerpt :Posteriorly, in the hantavirus-endemic area of Northwestern Argentina the LAN virus was recovered from human cases and from Calomys callosus samples (Levis et al., 2004). Recently, phylogenetic analysis of partial sequences of the N gene showed LAN virus as the cause of HCPS, and the possible association of the organism with C. callidus rodents in western Brazil (de Barros Lopes et al., 2014; Raboni et al., 2009a; Travassos da Rosa et al., 2012). The RIOMV was firstly described in Bolivia associated with O. microtis (Bharadwaj et al., 1997).
Hantaviruses and cardiopulmonary syndrome in South America
2014, Virus ResearchCitation Excerpt :Curiously, RIOMV was not detected in the saliva of the animals (unpublished data, 2013). LANV virus is harbored by Calomys callidus, a rodent that lives in the midwestern region of Brazil in a landscape of Cerrado and Amazon forest (Raboni et al., 2009). This region has been recently undergoing deforestation for extensive farming of soybean and other cereals.
Phylogenetic analysis of the S segment from Juquitiba hantavirus: Identification of two distinct lineages in Oligoryzomys nigripes
2013, Infection, Genetics and EvolutionCitation Excerpt :Following the emphasis of public health policies on reservoir surveillance (Mills and Childs, 1998), eco-epidemiological studies have been conducted in Brazilian regions where HCPS cases have occurred, and eight different hantavirus genotypes have been identified. Five of them have been associated with HCPS, namely Juquitiba (JUQV)/Araucária, Araraquara, Castelo dos Sonhos, Anajatuba and Laguna Negra, carried by Oligoryzomys nigripes, Necromys lasiurus, Oligoryzomys utiaritensis, Oligoryzomys fornesi and Calomys callidus, respectively (Monroe et al., 1999; Johnson et al., 1999; Suzuki et al., 2004; Raboni et al., 2009; Travassos da Rosa et al., 2010, 2011, 2012). Three more species, Rio Mearim, Jabora and a hantavirus related to the Seoul virus have also been identified in the rodent species Holochilus sciureus, Akodon montensis, and Rattus norvegicus, respectively, but their role in human disease has not yet been clarified (LeDuc et al., 1985; Rosa et al., 2005; de Oliveira et al., 2011).
Production and characterization of monoclonal antibodies against the recombinant nucleoprotein of Araucaria hantavirus
2009, Journal of Virological Methods