ResearchActive hexose correlated compound activates immune function to decrease bacterial load in a murine model of intramuscular infection
Section snippets
Bacteria
Klebsiella pneumoniae strain ATCC 43816 was obtained from American Type Culture Collection (Bethesda, MD). Stock cultures of the bacterium were maintained in tryptic soy broth (TSB) medium plus 50% glycerol and stored at −80°C until use.
Animals
Specific pathogen–free female Swiss/Webster mice (9–11 wk, each weighing 21–25 g) were purchased from Harlan Sprague-Dawley Laboratories (Indianapolis, IN). Animals were housed in a quiet, isolated room with controlled temperature, light cycle, and having access
Oral administration of AHCC promotes the clearance of bacteria from blood and organs in food-deprived and K pneumoniae–infected mice
We have shown in previous studies that AHCC increases the survival of mice in a rodent model of intramuscular infection [9]. To assess the role of AHCC on the progress of infection in this model, AHCC-treated and control mice were infected with sublethal doses of K pneumoniae, and the presence of bacteria was determined in blood and organs in successive days after infection. Fig. 1 shows the kinetics of bacterial organ load of food-deprived and infected mice receiving either AHCC or the
Comments
One of the most important aspects in the care of surgical patients continues to be the prevention and diligent control of microbial contamination. Among postoperative complications, infection constitutes the major limiting factor for recovery of patients after surgery or severe trauma [41]. Malnutrition is an important risk factor and has been associated with a higher incidence of postoperative infections [18], [42], [43]. Abstinence from food and drink before and after surgery is a common
Acknowledgments
This work was funded by a grant from the Amino Up Chemical Company (Sapporo, Japan).
The authors thank Hiroshi Nishioka for his comments and critical review of the manuscript.
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