Size and steroid-binding characterization of membrane-associated glucocorticoid receptor in S-49 lymphoma cells
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Cited by (46)
Receptor Transduction Pathways Mediating Hormone Action
2020, Sperling Pediatric Endocrinology: Expert Consult - Online and PrintGlucocorticoid receptor selectively mediates stress-induced suppression of innate immunity in the House Sparrow, Passer domesticus
2018, Developmental and Comparative ImmunologyCitation Excerpt :In addition, CORT can bind to plasma membrane receptors (Gametchu, 1987; Moore and Orchinik, 1994; Breuner and Orchinik, 2009; Espinoza et al., 2017). Intracellular GR can associate with the plasma membrane (reviewed in Groeneweg et al., 2012) and no membrane receptor for glucocorticoids with binding kinetics (birds, Breuner and Orchinik, 2001; Breuner and Orchinik, 2009; Schmidt et al., 2010; rats, Xiao et al., 2010; Rough-skinned Newt, Taricha granulosa, Orchinik et al., 1991) and molecular weight (mice; Gametchu et al., 1991) differing from those of classic GRs has been identified to date. We thus refer to the membrane receptor as the membrane-bound corticosteroid receptor (CR; reviewed in Groeneweg et al., 2012).
Further evidence for a membrane receptor that binds glucocorticoids in the rodent hypothalamus
2016, SteroidsCitation Excerpt :Specific [3H]-Cort binding in neuronal membranes in the newt was found to be about 85% of total binding, which is consistent with the higher membrane binding affinity in the newt brain [11]. Also consistent with lower-affinity glucocorticoid receptors in mammalian cell membranes, membrane binding sites for Dex in rat liver were shown to have a Kd value of approximately 400 nM [45], and studies using S-49 mouse lymphoma cells and human CCRF-CEM acute lymphoblastic leukemia cells [29,30] revealed a glucocorticoid Kd value of 239 nM for the membrane fraction compared to 19.5 nM for the cytosolic fraction [46]. Our findings here of Kd values of 30–42 nM suggest an approximately 10-fold higher affinity of glucocorticoid receptors in hypothalamic cell membranes.
Dissection of glucocorticoid receptor-mediated inhibition of the hypothalamic-pituitary-adrenal axis by gene targeting in mice
2015, Frontiers in NeuroendocrinologyCitation Excerpt :One mode of GR action occurs through non-genomic signaling pathways, that produce fast-acting effects in the order of seconds to a few minutes (de Kloet et al., 2008; Groeneweg et al., 2011a). This occurs through GC action on membrane bound receptors or cytoplasmic GR that activate rapid signaling of second messenger pathways (Bartholome et al., 2004; Cato et al., 2002; Gametchu et al., 1991; Spies et al., 2011; Zhang et al., 2012). The other mode is the classical GR function which occurs when activated GR translocates into the nucleus and functions as a transcription factor to modulate genomic signaling pathways, which can take a period of 15 min to several hours.
Receptor transduction pathways mediating hormone action
2014, Pediatric Endocrinology: Fourth EditionMembrane glucocorticoid receptor activation induces proteomic changes aligning with classical glucocorticoid effects
2013, Molecular and Cellular Proteomics
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Present address: University of Texas Medical Branch, Galveston, Texas, 77550.