Theoretical studies of enzymic reactions: Dielectric, electrostatic and steric stabilization of the carbonium ion in the reaction of lysozyme

https://doi.org/10.1016/0022-2836(76)90311-9Get rights and content

Abstract

A general method for detailed study of enzymic reactions is presented. The method considers the complete enzyme-substrate complex together with the surrounding solvent and evaluates all the different quantum mechanical and classical energy factors that can affect the reaction pathway. These factors include the quantum mechanical energies associated with bond cleavage and charge redistribution of the substrate and the classical energies of steric and electrostatic interactions between the substrate and the enzyme. The electrostatic polarization of the enzyme atoms and the orientation of the dipoles of the surrounding water molecules is simulated by a microscopic dielectric model. The solvation energy resulting from this polarization is considerable and must be included in any realistic calculation of chemical reactions involving anything more than an isolated molecule in vacuo. Without it, acidic groups can never become ionized and the charge distribution on the substrate will not be reasonable. The same dielectric model can also be used to study the reaction of the substrate in solution. In this way the reaction in solution can be compared with the enzymic reaction.

In this paper we study the stability of the carbonium ion intermediate formed in the cleavage of a glycosidic bond by lysozyme. It is found that electrostatic stabilization is an important factor in increasing the rate of the reaction step that leads to the formation of the carbonium ion intermediate. Steric factors, such as the strain of the substrate on binding to lysozyme, do not seem to contribute significantly.

References (39)

  • R.D. Diamond

    J. Mol. Biol

    (1974)
  • L.O. Ford et al.

    J. Mol. Biol

    (1974)
  • M. Levitt

    J. Mol. Biol

    (1974)
  • G.H. Loew et al.

    J. Theoret. Biol

    (1972)
  • J.M. McIver et al.

    Chem. Phys. Lett

    (1971)
  • H. Umeyama et al.

    J. Theoret. Biol

    (1973)
  • D.M. Blow et al.

    Nature (London)

    (1969)
  • C.C.F. Blake et al.
  • M. Born

    Z. Physik

    (1920)
  • D.M. Chipman et al.

    Science

    (1969)
  • F.W. Dahlquist et al.

    Biochemistry

    (1969)
  • R. Daudel et al.
  • M. De Paz et al.

    J. Chem. Phys

    (1970)
  • M.J.S. Dewar et al.

    J. Amer. Chem. Soc

    (1970)
  • R.E. Dickerson et al.
  • S. Doonan et al.

    Prog. Biophys. Mol. Biol

    (1970)
  • H.L. Friedman et al.
  • L.G. Hepler et al.
  • Cited by (0)

    View full text