Function of adenosine triphosphate in the activation of luciferin☆
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Cited by (61)
Characterisation of utrophin modulator SMT C1100 as a non-competitive inhibitor of firefly luciferase
2020, Bioorganic ChemistryCitation Excerpt :Relaxation to the ground state of oxyluciferin releases a photon of light of wavelength 560–610 nm [16]. FLuc can also perform ‘dark’ reactions such as oxidation of the intermediate LH2-AMP to form the potent tight-binding inhibitor l-AMP 5 which is responsible for the flashing of firefly lanterns [11,14,17]. FLuc inhibitors are typically linear, planar small molecules and some related structure–activity-relationships have been established in the literature [4,11,18].
Firefly Luciferase as Biocatalysts
2017, Future Directions in Biocatalysis: Second EditionEthanol's Action Mechanisms in the Brain: From Lipid General Alterations to Specific Protein Receptor Binding
2017, Addictive Substances and Neurological Disease: Alcohol, Tobacco, Caffeine, and Drugs of Abuse in Everyday LifestylesPoint mutations in firefly luciferase C-domain demonstrate its significance in green color of bioluminescence
2014, Biochimica et Biophysica Acta - Proteins and ProteomicsCitation Excerpt :They are frequently used as reporter genes [11], and various ATP assays [12] are based on the luciferase reaction. The luciferase catalytic reaction proceeds in two steps: 1) formation of the luciferyl adenylate, 2) and its further oxidation to the electronically excited form of the oxyluciferin (LO*) [13]. The relaxation of LO* is accompanied by the emission of visible light (λmax is in the range of 536–623 nm) [14] with an exceptionally high quantum yield [15].
Enantiodifferentiation of ketoprofen by Japanese firefly luciferase from Luciola lateralis
2011, Journal of Molecular Catalysis B: EnzymaticCitation Excerpt :Firefly luciferase catalyzes the oxidation of d-luciferin with molecular oxygen in the presence of ATP and Mg2+, producing light [1–3].
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Contribution No. 150 from the McCollum-Pratt Institute. Supported in part by grant from the National Science Foundation.