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Rutin Protects Dopaminergic Neurons from Oxidative Stress in an Animal Model of Parkinson’s Disease

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Abstract

This study was undertaken to investigate the neuroprotective effects of rutin (vitamin P) on 6-hydroxydopamine (6-OHDA)-induced Parkinson’s disease (PD) in rats. Oxidative stress and inflammation is an important event, play a crucial role in neurodegenerative diseases. Rutin has been shown to have antioxidant and anti-inflammatory actions, and thus was tested for its beneficial effects using 6-OHDA-induced PD rat model. Male Wistar rats were pre-treated with rutin (25 mg/kg bwt, orally) for 3 weeks and subjected to unilateral intrastriatal injection of 6-OHDA (10 μg in 0.1% ascorbic acid in normal saline). Three weeks after 6-OHDA infusion, rats were tested for neurobehavioral activity, and were killed after 4 weeks of 6-OHDA infusion for the estimation of thiobarbituric acid reactive substances, glutathione, and its dependent enzymes (glutathione peroxidase and glutathione reductase), dopamine (DA) and its metabolite 3,4-dihydroxyphenyl acetic acid. The increase in 6-OHDA-induced rotations and deficits in locomotor activity and motor coordination and decrease in antioxidant level, DA content and its metabolite and increase in the number of dopaminergic D2 receptors in striatum were protected significantly with lesioned group pre-treated with rutin. These findings were further supported by the histopathological and immunohistochemical findings in the substantia nigra that showed that rutin protected neurons from deleterious effects of 6-OHDA. These results suggest that the consumption of rutin, which is novel vitamin, may have the possibility of protective effect against the neurological disorder such as PD.

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Acknowledgments

The authors thank the Department of Ayurveda, Yoga & Naturopathy, Unani, Siddha and Homoeopathy (AYUSH), Ministry of Health and Family Welfare, Government of India, New Delhi, for financial assistance. We greatly acknowledge Ms. Lorie Leo, Department of Internal Medicine, University of Iowa, for reviewing and editing this manuscript. Technical assistance of Dharamvir Singh is gratefully appreciated.

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Authors and Affiliations

  1. Neurotoxicology Laboratory, Department of Medical Elementology & Toxicology, Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi, 110062, India

    Mohd. Moshahid Khan, Syed Shadab Raza, Hayate Javed, Ajmal Ahmad, Andleeb Khan & Fakhrul Islam

  2. Department of Biotechnology, Faculty of Pharmacy, Jamia Hamdard (Hamdard University), Hamdard Nagar, New Delhi, 110062, India

    Farah Islam

  3. Department of Neurology, Carver College of Medicine, University of Iowa, Iowa, IA, USA

    Mohd. Moshahid Khan

  4. Department of Neurology, Georgia Health Science University, Augusta, GA, USA

    Ajmal Ahmad

  5. Neuroscience and Toxicology Unit, Faculty of Pharmacy, Jazan University, Gizan, Kingdom of Saudi Arabia

    Mohammed M. Safhi & Fakhrul Islam

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  1. Mohd. Moshahid Khan
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  2. Syed Shadab Raza
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Correspondence to Fakhrul Islam.

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Neurotoxicology Laboratory—Fund for the Improvement of Science and Technology Sponsored by DST and Special Assistance Programme Sponsored by UGC.

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Moshahid Khan, M., Raza, S.S., Javed, H. et al. Rutin Protects Dopaminergic Neurons from Oxidative Stress in an Animal Model of Parkinson’s Disease. Neurotox Res 22, 1–15 (2012). https://doi.org/10.1007/s12640-011-9295-2

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