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The Current State of Chromatin Immunoprecipitation

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Abstract

The biological significance of interactions of nuclear proteins with DNA in the context of gene expression, cell differentiation, or disease has immensely been enhanced by the advent of chromatin immunoprecipitation (ChIP). ChIP is a technique whereby a protein of interest is selectively immunoprecipitated from a chromatin preparation to determine the DNA sequences associated with it. ChIP has been widely used to map the localization of post-translationally modified histones, histone variants, transcription factors, or chromatin modifying enzymes on the genome or on a given locus. In spite of its power, ChIP has for a long time remained a cumbersome procedure requiring large numbers of cells. These limitations have sparked the development of modifications to shorten the procedure, simplify sample handling and make ChIP amenable to small numbers of cells. In addition, the combination of ChIP with DNA microarray and high-throughput sequencing technologies has in recent years enabled the profiling of histone modification, histone variants, and transcription factor occupancy on a genome-wide scale. This review highlights the variations on the theme of the ChIP assay, the various detection methods applied downstream of ChIP, and examples of their application.

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Acknowledgments

Our work is supported by grants from the Research Council of Norway, the Norwegian Cancer Society and the University of Oslo.

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  1. Institute of Basic Medical Sciences, Faculty of Medicine, Norwegian Center for Stem Cell Research, University of Oslo, Blindern, PO Box 1112, 0317, Oslo, Norway

    Philippe Collas

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Correspondence to Philippe Collas.

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Collas, P. The Current State of Chromatin Immunoprecipitation. Mol Biotechnol 45, 87–100 (2010). https://doi.org/10.1007/s12033-009-9239-8

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  • DOI: https://doi.org/10.1007/s12033-009-9239-8

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