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Inhibitory Effects of Ginkgo biloba Extract on Inflammatory Mediator Production by Porphyromonas gingivalis Lipopolysaccharide in Murine Macrophages via Nrf-2 Mediated Heme Oxygenase-1 Signaling Pathways

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Abstract

Periodontitis is an oral chronic inflammatory disease that influences systemic diseases. Heme oxygenase-1 has several beneficial abilities through Nrf-2 regulation. Ginkgo biloba has been reported to have anti-inflammatory effects associated with heme oxygenase-1 (HO-1) expression. In this study, we investigated whether the anti-inflammatory effects of G. biloba were involved with Nrf-2-mediated HO-1 expression in Porphyromonas gingivalis LPS-stimulated RAW264.7 macrophage cells. G. biloba was extracted with ethyl acetate (EGB). EGB exhibited anti-inflammatory activities, which suppressed the production of pro-inflammatory mediators, the activation of mitogen-activated protein kinases, and nuclear translocation of transcription factors. EGB also up-regulated the HO-1 expression, and the Nrf-2 level in the nucleus and its transactivity. Furthermore, reduced pro-inflammatory mediator levels by EGB were inverted in the presence of SnPP. The collective results suggest that the anti-inflammatory effects of EGB are due to the HO-1 expression via up-regulation of Nrf-2 in RAW 264.7 cells stimulated by P. gingivalis LPS.

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Authors and Affiliations

  1. BIO-IT Fusion Technology Research Institute, Pusan National University, Busan, 609-735, South Korea

    Eun Yeon Ryu, Sun Young Park & Young Hun Kim

  2. Department of Microbiology, Pusan National University, Busan, 609-735, South Korea

    Ah Jeong Park, Sung Hae Park, Hye Won Eom & Sang-Joon Lee

  3. Institute for Research & Industry Cooperation, Pusan National University, Busan, 609-735, South Korea

    Geuntae Park

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  1. Eun Yeon Ryu
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  2. Ah Jeong Park
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  3. Sun Young Park
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Correspondence to Sang-Joon Lee.

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E.Y. Ryu and A.J. Park have contributed equally to this work.

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Ryu, E.Y., Park, A.J., Park, S.Y. et al. Inhibitory Effects of Ginkgo biloba Extract on Inflammatory Mediator Production by Porphyromonas gingivalis Lipopolysaccharide in Murine Macrophages via Nrf-2 Mediated Heme Oxygenase-1 Signaling Pathways. Inflammation 35, 1477–1486 (2012). https://doi.org/10.1007/s10753-012-9461-6

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  • DOI: https://doi.org/10.1007/s10753-012-9461-6

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