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Bioprocessing of plant cell cultures for mass production of targeted compounds

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Abstract

More than a century has passed since the first attempt to cultivate plant cells in vitro. During this time, plant cell cultures have become increasingly attractive and cost-effective alternatives to classical approaches for the mass production of plant-derived metabolites. Furthermore, plant cell culture is the only economically feasible way of producing some high-value metabolites (e.g., paclitaxel) from rare and/or threatened plants. This review summarizes recent advances in bioprocessing aspects of plant cell cultures, from callus culture to product formation, with particular emphasis on the development of suitable bioreactor configurations (e.g., disposable reactors) for plant cell culture-based processes; the optimization of bioreactor culture environments as a powerful means to improve yields; bioreactor operational modes (fed-batch, continuous, and perfusion); and biomonitoring approaches. Recent trends in downstream processing are also considered.

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Acknowledgements

We thank Dr. V. Georgiev for preparing Fig. 1, and Profs. J.J. Zhong (Shanghai Jiao Tong University), Y. Xu, and X. Qian (East China University of Science and Technology, Shanghai, China) for providing us with the relative prices of the novel elicitors synthesized and used in their laboratories. Special thanks goes to D. Ullisch, R. Huber, and Prof. Dr. J. Büchs (chair of Biochemical Engineering, Aachen University of Technology, Germany) for providing us with the MTP cultivations data. This work has been supported by a National Science Fund of Bulgaria under contract number DO 02-261/2008.

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  1. Department of Microbial Biosynthesis and Biotechnologies, Laboratory in Plovdiv, Institute of Microbiology, Bulgarian Academy of Sciences, 26 Maritza Blvd, 4002, Plovdiv, Bulgaria

    Milen I. Georgiev

  2. Institute of Food Technology and Bioprocess Engineering, Dresden University of Technology, 01069, Dresden, Germany

    Jost Weber

  3. UMR CNRS 8076 BioCIS, Laboratory of Natural Products Chemistry, School of Pharmacy, University of Paris-Sud 11, 92296, Châtenay-Malabry, France

    Alexandre Maciuk

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  1. Milen I. Georgiev
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Correspondence to Milen I. Georgiev.

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This paper is dedicated to Prof. Dr. Mladenka P. Ilieva on the occasion of her 70th birthday.

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Georgiev, M.I., Weber, J. & Maciuk, A. Bioprocessing of plant cell cultures for mass production of targeted compounds. Appl Microbiol Biotechnol 83, 809–823 (2009). https://doi.org/10.1007/s00253-009-2049-x

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