Abstract
A Gram-negative, aerobic, and long rod-shaped bacterium, designated as H33E-04T, was isolated from the soil of reclaimed land, Republic of Korea. The strain grew at a temperature range of 15–40 °C, pH 5.0–10.0, and 0–2% NaCl (w/v). The phylogenetic analysis based on 16S rRNA gene sequences showed that strain H33E-04T was in the same clade with Chitinophaga pinensis DSM 2588T, Chitinophaga filiformis IFO 15056T, and Chitinophaga ginsengisoli Gsoil 052T with 98.4%, 97.9%, and 97.8% sequence similarities, respectively. The de novo genome assembly revealed that the DNA G + C content of the strain was 46.2 mol%. Comparative genome analysis between strain H33E-04T and C. pinensis DSM 2588 T showed that the average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values were 79.9% and 23.4%, respectively. The major respiratory quinone was menaquinone-7 (MK-7) and the predominant cellular fatty acids were iso-C15:0 (31.7%), C16:1 ω5c (31.2%), and iso-C17:0 3-OH (11.8%), supporting the affiliation of strain H33E-04T with the genus Chitinophaga. Based on phylogenetic, physiological, and chemotaxonomic characteristics, strain H33E-04T represents a novel species of the genus Chitinophaga, for which the name Chitinophaga agri sp. nov. is proposed. The type strain of Chitinophaga agri is H33E-04T (= KACC 21303T = NBRC114512T).
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This study was carried out with the support of “Research Program for Agricultural Science & Technology Development (Project no. PJ01424401)” from the National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.
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The GenBank accession numbers for the 16S rRNA gene sequence and the genome sequence of strain H33E-04T are MN998543 and CP048113, respectively.
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Lee, S.A., Heo, J., Kim, TW. et al. Chitinophaga agri sp. nov., a bacterium isolated from soil of reclaimed land. Arch Microbiol 203, 809–815 (2021). https://doi.org/10.1007/s00203-020-02066-9
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DOI: https://doi.org/10.1007/s00203-020-02066-9