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The effects of lazaroid U-74389G in a rat sepsis model

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Abstract

Objective and design

To examine the protective effects of a lazaroid, 21-aminosteroid U-74389G, in a rat septic shock model.

Materials or subjects

Male Sprague–Dawley rats (n = 60) aged 6–8 months.

Treatment

Groups were exposed to 500 cGy radiation followed by E. coli inoculation, and either placebo or lazaroid injection (10 mg/kg intraperitoneal) 5 days after irradiation.

Methods

Hemodynamic measurements, arterial blood gases, serum lactate, total antioxidative capacity, and cytokine levels were measured at specific time intervals.

Results

Treatment with the lazaroid U-74389G maintained cardiac output and mean aortic pressure. Lazaroid treatment also prevented the increase in serum lactate seen in placebo-treated rats. Cytokine serum levels in lazaroid-treated rats were not significantly different from those in placebo-treated rats at any time point.

Conclusions

Lazaroid treatment of E. coli-inoculated septic animals lessens the hemodynamic deterioration seen in sepsis.

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Acknowledgments

We thank Chi-Huei Chang, Hsin-Hui Tseng, and Yang-Ming Tseng for contributing animal care, linear accelerator operation, and laboratory examinations. The grant was supported by Kaohsiung Veterans General Hospital, VGHKS 95-064.

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Authors and Affiliations

  1. Department of Emergency Medicine, Kaohsiung Veterans General Hospital, 386, Ta-Chung 1st Road, Kaohsiung City, Taiwan

    Yun-Te Chang, Shue-Ren Wann & Hong-Tai Chang

  2. Department of Pediatrics, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan

    Kai-Hseng Hsieh

  3. The Infectious Diseases Section, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan

    Yung-Ching Liu

  4. Department of Radiation Oncology, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan

    Ching-Hsiung Chang

  5. School of Medicine, National Yang-Ming University, Taipei, Taiwan

    Yun-Te Chang, Mu-Shun Huang & Chun-I Huang

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  1. Yun-Te Chang
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  2. Shue-Ren Wann
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Correspondence to Shue-Ren Wann.

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Responsible Editor: Artur Bauhofer.

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Chang, YT., Wann, SR., Hsieh, KH. et al. The effects of lazaroid U-74389G in a rat sepsis model. Inflamm. Res. 60, 29–35 (2011). https://doi.org/10.1007/s00011-010-0231-3

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  • DOI: https://doi.org/10.1007/s00011-010-0231-3

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