Skip to main content
Log in

Comparison of the growth of virulent and attenuated strains of Candida albicans in the kidneys of normal and cortisone-treated mice by Chitin assay

  • Published:
Mycopathologia Aims and scope Submit manuscript

Abstract

Candida albicans strain 22114 was avirulent for mice compared with strain 19321 in that, administered intravenously, 106 blastospores of 22114 failed to kill whereas 106 blastospores of 19321 produced 100% mortality. Cortisone treatment rendered mice susceptible to killing by 22114. Chitin assay showed that cortisone stimulated the growth of both strains in the mouse kidney. Growth of 19321 was increased up to five-fold and 22114 up to forty-fold. The strains may have differential susceptibility to cortisone-sensitive host defences which control fungal growth in vivo.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Armstrong, D. 1976. Candida infections. Practice of Medicine 3: Chptr. 61.

  2. Chattaway, F.W., M.R. Holmes & A.J.E. Barlow, 1968. Cell wall composition of mycelial and blastospore forms of Candida albicans. J. Gen. Microbiol. 51: 367–376.

    Google Scholar 

  3. Evans, Z.A. & D.N. Mardon, 1977. Organ localization in mice challenged with a typical Candida albicans strain and a pseudohyphal variant (39780). Proc. Soc. Exp. Biol. Med. 155: 234–238.

    Google Scholar 

  4. Harrower, K.M. 1977. Estimation of resistance of three wheat cultivars to Septoria tritici using a chemical method for determination of fungal mycelium. Trans. Brit. Mycol. Soc. 69: 15–19.

    Google Scholar 

  5. Lehmann, P.F. & L.O. White, 1975. Chitin assay used to demonstrate renal localisation and cortisone-enhanced growth of Aspergillus fumigatus mycelium in mice. Infect. Immun. 12: 987–992.

    Google Scholar 

  6. Lehrer, R.I. & M.J. Cline, 1969. Interaction of Candida albicans with human leukocytes and serum. J. Bacteriol. 98: 996–1004.

    Google Scholar 

  7. Louria, D.B., R.G. Brayton & G. Finkel, 1963. Studies on the pathogenesis of experimental Candida albicans infection in mice. Sabouraudia 2: 271–276.

    Google Scholar 

  8. Ride, J.P. & R.B. Drysdale, 1972. A rapid method for the chemical estimation of filamentous fungi in plant tissues. Physiol. Plant. Pathol. 2: 7–15.

    Google Scholar 

  9. Rogers, T. & E. Balish, 1976. Experimental Candida albicans infection in conventional mice and germ-free rats. Infect. Immun. 14: 33–38.

    Google Scholar 

  10. Sidransky, H., S.M. Epstein, E. Verney & C. Horowitz, 1972. Experimental visceral aspergillosis. Amer. J. Pathol. 69: 55–68.

    Google Scholar 

  11. Singer, C., M.H. Kaplan & D. Armstrong, 1977. Bacteremia and fungemia complicating neoplastic disease. Amer. J. Med. 62: 731–742.

    Google Scholar 

  12. Ward, P.A. 1966. The chemosuppression of chemotaxis. J. Exptl. Med. 124: 209–226.

    Google Scholar 

  13. White, L.O., H.C. Newham & J.P. Ride, 1978. Estimation of Absidia ramosa infection in the brain and kidneys of cortisone-treated mice by chitin assay. Mycopathologia 63: 177–179.

    Google Scholar 

  14. White, L.O., M.D. Richardson, H. Newham, E. Gibb & R.C. Warren, 1977. Circulating antigen of Aspergillus fumigatus in cortisone-treated mice challenged with conidia: detection by counterimmuno-electrophoresis. F.E.M.S. Microbiol. Letts. 2: 153–156.

    Google Scholar 

  15. Winblad, B. 1976. Experimental renal candidiasis in mice and guinea pigs. Acta. Pathol. Microbiol. Scand. A83: 406–414.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

White, L.O., Gibb, E., Newham, H.C. et al. Comparison of the growth of virulent and attenuated strains of Candida albicans in the kidneys of normal and cortisone-treated mice by Chitin assay. Mycopathologia 67, 173–177 (1979). https://doi.org/10.1007/BF00470753

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00470753

Keywords

Navigation