Abstract
The complete amino acid sequence of the major component of hemoglobin from amur-leopard (Panthera pardus orientalis) is presented. The major component accounts for more than 90% of the total hemoglobin. Separation of the globin subunits was achieved by ion-exchange chromatography on CM-cellulose in urea. The sequence was studied by automatic Edman degradation of tryptic and hydrolytic peptides. Alignment was carried out with human hemoglobin sequence. The β NH2 terminus is blocked with Ac-serine. The data are compared with other mammalian hemoglobins and results are discussed with respect to sequence and physiology.
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Alter, B. P., Goff, S. C., Efremov, G. D., Gravely, M. E. and Huisman, T. H. J. (1980).Br. J. Haematol. 44, 527–534.
Braunitzer, G., Schrank, B. and Ruhfus, A. (1970).Hoppe-Seyler's Z. Physiol. Chem. 351, 1589–1590.
Braunitzer, G., Schrank, B., Ruhfus, A., Petersen, S., and Petersen, U. (1971).Hoppe-Seyler's Z. Physiol. Chem. 352, 1730–1732.
Braunitzer, G., Schrank, B., Stangl, A., and Scheithauer, U. (1978).Hoppe-Seyler's Z. Physiol. Chem. 359, 137–146.
Brimhall, B., Stenzel, P., Dresler, S. L., Hermodson, M., Stangland, K., and Joyce, J. (1977).J. Mol. Evol. 9, 237–260.
Clegg, J. B., Naughton, M. A., and Weatherall, D. J. (1966).J. Mol. Biol. 19, 91–108.
Edman, P. and Begg, B. (1967).Eur. J. Biochem. 1, 80–91.
Hirs, C. H. W. (1956).J. Biol. Chem. 219, 619–621.
Jauregui-Adell,et al. (1975).
Kratzin, H., Yang, C., Krusche, J. U., and Hilschman, N. (1980).Hoppe-Seyler's Z. Physiol. Chem. 361, 1591–1598.
Lottspeich, F. (1980).Hoppe-Seyler's Z. Physiol. Chem. 361, 1829–1834.
Maurer, H. R. (1971).Disc Electrophoresis, de Gruyter, Berlin.
Morris, D. (1964).The Mammal, Harper & Row, New York.
Perutz, M. F., and Imai, K. (1980).J. Mol. Biol. 136, 183–191.
Pfletschinger, J., and Braunitzer, G. (1980).Hoppe-Seyler's Z. Physiol. Chem. 361, 925–931.
Rossi-Fanelli, A., and Antonini, E. (1958).Biochim. Biophys. Acta30, 608–615.
Taketa, F. (1973).Comp. Biochem. Physiol. 45B, 813–823.
Taketa, F. (1974).N.Y. Acad. Sci. 241, 524–537.
Taketa, F., Mauk, A. G., and Lessard, J. L. (1971).J. Biol. Chem. 246, 4471–4476.
Taketa, F., Attermeier, M. H., and Mauk, A. G. (1972).J. Biol. Chem. 247, 33–35.
Zimmermann, C. L., and Pisano, J. (1977).Meth. Enzymol. 57, 45–51.
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85th communication on hemoglobin.
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Abbasi, A., Braunitzer, G. The primary structure of hemoglobin from amur-leopard (Panthera pardus orientalis). J Protein Chem 4, 57–67 (1985). https://doi.org/10.1007/BF01025493
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DOI: https://doi.org/10.1007/BF01025493