Summary
Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils. Using a panel of human-rodent cell hybrids that preferentially segregate human chromosomes and in situ hybridization, the MDNCF/IL-8 gene was placed on the human gene map at position 4q12-q21. This is the same location where at least three other members (platelet factor 4, melanoma growth stimulatory activity, and interferon-γ induced factor) of the platelet factor 4 gene superfamily reside. In addition, a restriction fragment length polymorphism was identified using MDNCF as a probe in screening genomic DNA digested with HindIII from unrelated individuals.
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References
Begg GS, Pepper DS, Chesterman CN, Morgan FJ (1978) Complete covalent structure of human β-thromboglobulin. Biochemistry 17:1739–1744
Dean M, O'Connell P, Leppert M, Park M, Amos JA, Phillips DG, White R, Vande Woude GF (1987) Three additional DNA polymorphisms in the met gene and D7S8 locus: use in prenatal diagnosis fo cystic fibrosis. J Pediatr 4:490–495
Deuel TF, Senior RM, Chang D, Griffin GL, Heinrikson RL, Kaiser ET (1981) Platelet factor 4 is chemotactic for neutrophils and monocytes. Proc Natl Acad Sci USA 78:4584–4587
Griffin CA, Emanuel BS, LaRocco P, Schwartz E, Poncz M (1987) Human platelet factor 4 gene is mapped to 4q12→q21. Cytogenet Cell Genet 45:67–69
Holt JC, Harris ME, Holt AM, Lange E, Henschen A, Niewiarowski S (1986) Characterization of human platelet basic protein, a precursor form of low-affinity platelet factor 4 and β-thromboglobulin. Biochemistry 25:1988–1996
Larsen CG, Anderson AO, Appella E, Oppenheim JJ, Matsushima K (1989) The neutrophil activating protein (NAP-1) is also chemotactic for T lymphocytes. Science 243:1464–1466
Luster AD, Unkeless JC, Ravetch JV (1985) γ-Interferon transcriptionally regulates an early-response gene containing homology to platelet proteins. Nature 315:672–676
Luster AD, Jhanwar SC, Chaganti RSK, Kersey JH, Ravetch JV (1987) Interferon inducible gene maps to a chromosomal band associated with a (4; 11) translocation in acute leukemia cells. Proc Natl Acad Sci USA 84:2868–2871
Marcum JA, McKenney JB, Rosenberg RD (1984) Acceleration of thrombin-antithrombin complex formation in rat hind quarters via heparin like molecules bound to the endothelium. J Clin Invest 74:341–350
Matsushima K, Morishita K, Yoshimura T, Lavu S, Kobayashi Y, Lew W, Appella E, Kung HF, Leonard EJ, Oppenheim JJ (1988) Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8) and the induction of MDNCF mRNA by interleukin 1 and tumor necrosis factor. J Exp Med 167:1883–1893
Modi WS, Nash WG, Ferrari AC, O'Brien SJ (1987) Cytogenetic methodologies for gene mapping and comparative analyses in mammalian cell culture systems. Gene Anal Tech 4:75–85
Modi WS, Masuda A, Yamada M, Oppenheim JJ, Matsushima K, O'Brien SJ (1988) Chromosomal localization of the human interleukin 1α (IL-1α) gene. Genomics 2:310–314
Mukaida N, Shiroo M, Matsushima K (1989) Genomic structure of the human monocyte-derived neutrophil chemotactic factor IL-8. J Immunol 143:1366–1371
Obaru K, Hattori T, Yamamura Y, Takatsuki K, Nomiyama H, Maeda S, Shimada K (1989) A cDNA clone inducible in human tonsillar lymphocytes by a tumor promoter codes for a novel protein of the β-thromboglobulin superfamily. Mol Immunol 26:423–426
O'Brien SJ, Nash WG, Goodwin JL, Lowy DR, Chang EH (1983) Dispersion of the ras family of transforming genes to four different chromosomes in man. Nature 302:839–842
Richmond A, Lawson DH, Dixon DW (1982) Release of an autostimulatory growth factor by human malignant melanoma cells. Cold Spring Harbor Conference. Cell Prolif 9:885–897
Richmond A, Balentien E, Thomas HG, Flaggs G, Barton DE, Spiess J, Bordoni R, Francke U, Derynck R (1988) Molecular characterization and chromosomal mapping of melanoma growth stimulatory activity, a growth factor structurally related to β-thromboglobulin. EMBO J 7:2025–2033
Schroder J-M, Mrowietz U, Morita E, Christophers E (1987) Purification and partial biochemical characterization of a human monocyte-derived, neutrophil-activating peptide that lacks interleukin 1 activity. J Immunol 139:3474–3483
Schroder J-M, Mrowietz U, Christophers E (1988) Purification and partial biologic characterization of a human lymphocyte derived peptide with potent neutrophil stimulating activity. J Immunol 140:3534–3540
Sugano S, Stoeckle MY, Hanafusa H (1987) Transformation by Rous sarcoma virus induces a novel gene with homology to a mitogenic platelet protein. Cell 49:321–328
Walz A, Peveri P, Aschauer H, Baggiolini M (1987) Purification and amino acid sequencing of NAF, a novel neutrophil-activating factor produced by monocytes. Biochem Biophys Res Commun 149:755–761
White R, Woodward S, Leppert M, O'Connell P, Hoff M, Herbst J, Lalouel J-M, Dean M, Vande Woude G (1985) A closely linked genetic marker for cystic fibrosis. Nature 318:382–384
Yarden Y, Kuang WJ, Yang-Feng T, Coussens L, Munemitsu S, Dull TJ, Chen E, Schlessinger J, Francke U, Ullrich A (1987) Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. EMBO J 6:3341–3351
Yoshimura T, Matsushima K, Oppenheim JJ, Leonard EJ (1987) eutrophil chemotactic factor produced by lipopolysaccharide (LPS)-stimulated human blood mononuclear leukocytes: partial characterization and separation from interleukin 1 (IL-1). J Immunol 139:788–793
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On leave from Genetics Section, Instituto Nacional do Cancer (RJ)/ Department of Genetics, Universidade Federal do Rio de Janeiro, Brazil
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Modi, W.S., Dean, M., Seuanez, H.N. et al. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8) resides in a gene cluster along with several other members of the platelet factor 4 gene superfamily. Hum Genet 84, 185–187 (1990). https://doi.org/10.1007/BF00208938
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DOI: https://doi.org/10.1007/BF00208938