Summary
Lymphocyte-mediated lysis of autologous tumor cells (autologous lymphocyte cytotoxocity ALC) was tested at the time of surgery in 108 patients (46 squamous cell carcinomas of the lung, 25 adenocarcinomas of the lung, 19 soft tissue sarcomas and 18 osteosarcomas). The clinical course of these patients in relation to the test results has been published previously. The group was evaluated again after an extended observation time, now with a mean of 80.2 months (range 36–108). The test was rarely positive in patients with metastasis (2 out of 28 experiments).
There was a correlation between the ALC results and the postsurgical clinical course for patients without detectable metastasis in that (1) a negative test was invariably a bad prognostic sign, i.e., all 32 patients with negative ALC died within 3 years (mean survival time 16.1 months). (2) The remission and survival times were longer for the ALC positive patients (p<0.001). (3) All 37 individuals who are alive at present without recurrence belong to the reactive group.
The ALC results correlated with the clinical course in 88% of patients. The correlation was highest for the groups of soft tissue sarcoma and adenocarcinoma of the lung. There was no correlation between killing of K562 cells and ALC, or between lymphoproliferative response to PHA and ALC reactivity.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Broström LA, Aparisi T, Ingimarsson S, Lagergren C, Nilsonne U, Strander H, Söderberg G (1980) Can historical controls be used in current clinical trials in osteosarcoma? Metastases and survival in a historical and a concurrent group. Int J Radiat Oncol Biol Phys 6:1717
Colton T (1974) Statistics in medicine. Little Brown Boston, p. 244
Dean JH, Connor F, Herberman RB et al (1977) The relative proliferation index as a more sensitive parameter for evaluating lymphoproliferative responses of cancer patients to mitogens and alloantigens. Int J Cancer 20:359
Dean JH, Cannon GB, Jerrells TR, McCoy JL, Herberman RB (1980) Sensitive measurement of immunocompetence and anti-tumor reactivity in lung carcinoma patients and possible mechanisms of immunosuppression. In: Cripsen (ed) Neoplasm immunity: experimental and clinical., Elsevier North Holland Inc. pp 367–386
Enzinger FM (1977) Recent developments in the classification of soft tissue sarcomas. In. Management of primary bone and soft tissue tumors. M. D. Anderson Hospital and Tumor Institute, Year Book medical publishers, pp 219
Fossati G, Balsari A, Taramelli D, Sensi ML, Pellegris G, Nava M, Parmiani G (1982) Lysis of autologous human melanoma cells by in vitro allosensitised peripheral blood lymphocytes. Cancer Immunol Immunother 14:99
Strander H, Adamson U, Aparisi T, Broström L-A, Cantell K, Einhorn S, Hall K, Ingimarsson S, Nilsonne U, Söderberg G (1979) Adjuvant interferon treatment of human osteosarcoma. Recent Results Cancer Res, 68:40
Vánky F, and Klein E (1982) Specificity of auto-tumor cytotoxicity exerted by fresh, activated and propagated human T-lymphocytes. Int J Cancer 29:547
Vánky F, Vose BM, Fopp M, Klein E (1979) Human tumorlymphocyte interaction in vitro. VI. Specificity of primary and secondary autologous lymphocyte mediated cytotoxicity. J Natl Cancer Inst 62:1407
Vánky F, Péterffy A, Böök K, Willems J, Klein E, Klein G (1983) Correlation between lymphocyte-mediated auto-tumor reactivities and the clinical course: II. Evaluation of 69 patients with lung carcinoma. Cancer Immunol Immunother 16:17
Vánky F, Willems J, Kreicbergs A, Aparisi T, Andréen M, Broström L-A, Nilsonne U, Klein E, Klein G (1983) Correlation between lymphocyte mediated auto-tumor reactivities and the clinical course: I. Evaluation of 46 patients with sarcoma. Cancer Immunol Immunother 16:11
Vose BM (1980) Specific T-cell-mediated killing of autologous lung tumor cells. Cell Immunol 55:12
Vose BM, Vánky F, Fopp M and Klein E (1978) Restricted autologous lymphocytotoxicity in lung neoplasia. Br J Cancer 38:375
Vose BM, Callagher P, Moore M, Schofield PF (1982) Specific and non-specific lymphocyte cytotoxicity in colon carcinoma. Br J Cancer 44:846
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vánky, F., Klein, E., Willems, J. et al. Lysis of autologous tumor cells by blood lymphocytes tested at the time of surgery. Cancer Immunol Immunother 21, 69–76 (1986). https://doi.org/10.1007/BF00199380
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00199380