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Molecular arrangement of pigment-protein complex of photosystem 1

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Abstract

The circular dichroism (CD) method was applied to study the molecular organization of P700, antenna chlorophyll and protein of photosystem 1 complexes (CP1), isolated from chloroplasts under mild treatment with Triton X-100. Analysis of CD spectra and protein: chlorophyll: P700 ratios for CP1 complexes that were different in their chlorophyll content indicate that CP1 preparations can be considered as a mixture of CP1-RC, containing P700 (10–20%), and CP1-LH without P700 (80–90%). Both types of complexes contain approximately 25 chlorophyll molecules, and the destruction of their spatial organization with detergents represents a cooperative transition. The rate of chlorophyll destruction in CP1-LH is much higher than that in CP1-RC. In both complexes a 65 kDa polypeptide predominates, whose secondary structure (typical for α/β proteins) is stable to Triton X-100 and does not depends on the chlorophyll content. Chlorophyll seems to be grouped in clusters (5–7 molecules) in the hydrophobic cores of 2–3 parallel α/β domains of the 65 kDa protein. Only one of the clusters in CP1-RC includes P700; on P700 photooxidation the change of its interaction with the nearest pigment environment results in a complicated shape of the light-induced CD spectra.

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Abbreviations

PS1:

photosystem 1

CP1:

pigment-protein complex of PS1

Chl:

chlorophyll a

CP1-140:

CP1 with ratio Ch1:P700 140

RC:

reaction center

LH:

light-harvesting pigment

CP1-RC:

CP1, containing P700

CP1-LH:

CP1 without P700 (containing LH)

CD:

circular dichroism

SDS:

sodium dodecyl sulfate

References

  1. Anderson JM (1980) Biochim Biophys Acta 591:113–126

    Google Scholar 

  2. Bengis C and Nelson N (1977) J Biol Chem 252:4564–4569

    Google Scholar 

  3. Bolotina IA, Chekhov VO, Lugauskas VYu and Ptitsyn OB (1980) Mol Biol (USSR) 14:902–909

    Google Scholar 

  4. Bolotina IA, Chekhov VO, Lugauskas VYu and Ptitsyn OB (1981) Mol Biol (USSR) 15:167–175

    Google Scholar 

  5. Duysens LNM (1952) Doctoral thesis, University of Utrecht

  6. Hiyama T and Ke B (1972) Biochim Biophys Acta 261:160–172

    Google Scholar 

  7. Ikegami I and Ke B (1984) Biochim Biophys Acta 764:70–79

    Google Scholar 

  8. Karapetyan NV, Litvin FF and Krasnovsky AA (1963) Biofizika (USSR) 8:191–200

    Google Scholar 

  9. Karapetyan NV, Shubin VV, Rakhimberdieva MG, Vashchenko RG and Bolychevtseva YuV (1984) FEBS Lett 173:209–212

    Google Scholar 

  10. Krasnovksy AA, Kosobutskaya LM and Voinovskaya KK (1953) DAN USSR 92: 1201–1204

    Google Scholar 

  11. Mullet JR, Burke JJ and Arntzen CJ (1980) Plant Physiol 65:814–822

    Google Scholar 

  12. Nabedryk E, Biaudet P, Darr S, Arntzen CJ and Breton J (1984) Biochim Biophys Acta 767:640–647

    Google Scholar 

  13. Philipson KD, Sato VL and Sauer K (1972) Biochemistry 11:4591–4595

    Google Scholar 

  14. Richardson JS (1981) In: Anfinsen CB, Edsall JT and Richards PM (eds) Advances in protein chemistry, pp. 167–337. New York: Academic Press

    Google Scholar 

  15. Scops RK (1974) Anal Biochem 59:277–282

    Google Scholar 

  16. Shiozawa JA, Alberte RS and Thornber JF (1974) Arch Biochem Biophys 165: 388–397

    Google Scholar 

  17. Shubin VV, Efimovskaya TV and Karapetyan NV (1981) J Phys Chem (USSR) 55: 2916–2921

    Google Scholar 

  18. Shubin VV, Vaschenko RG and Karapetyan NV (1985) Mol Biol (USSR) 19: 841–850

    Google Scholar 

  19. Thornber JP (1975) Annu Rev Plant Physiol 26:127–158

    Google Scholar 

  20. Vierling E and Alberte RS (1983) Plant Physiol 72:625–633

    Google Scholar 

Download references

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Dedicated to Prof. L.N.M. Duysens on the occasion of his retirement

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Shubin, V.V., Karapetyan, N.V. & Krasnovsky, A.A. Molecular arrangement of pigment-protein complex of photosystem 1. Photosynth Res 9, 3–12 (1986). https://doi.org/10.1007/BF00029726

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  • DOI: https://doi.org/10.1007/BF00029726

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