Abstract
Exosomes are small extracellular vesicles that contain RNA, lipids, and proteins and can act as cellular messengers, carrying information to cells and tissues in the body. Thus, sensitive, label-free, and multiplexed analysis of exosomes may help in early diagnosis of important diseases. Here, we describe the process of pretreatment of cell-derived exosomes, preparation of surface-enhanced Raman scattering (SERS) substrates, and label-free SERS detection of exosomes using sodium borohydride aggregators. This method can enable the observation of SERS signals of exosomes that are clear and stable and have a good signal-to-noise ratio.
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Acknowledgments
HMU Marshal Initiative Funding (No.: HMUMIF-21012); National Natural Science Foundation for Youth (No.: 82202648); Jian-An Huang acknowledges the DigiHealth-project, a strategic profiling project at the University of Oulu that is supported by the Academy of Finland (project number 326291) and the University of Oulu.
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Li, Y., Wang, Y., Tian, J., Huang, JA. (2023). Detection of Cell-Derived Exosomes Via Surface-Enhanced Raman Scattering Using Aggregated Silver Nanoparticles. In: Vainio, S. (eds) Cell-Secreted Vesicles. Methods in Molecular Biology, vol 2668. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3203-1_2
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DOI: https://doi.org/10.1007/978-1-0716-3203-1_2
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Online ISBN: 978-1-0716-3203-1
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