Formulation and Characterization of Nystatin-loaded Nanostructured Lipid Carriers for Topical Delivery against Cutaneous Candidiasis
Rawia M. Khalil *
Department of Pharmaceutical Technology, National Research Centre, Dokki, CAiro, Egypt.
A. Abd-Elbary
Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Mahfouz A. Kassem
Department of Pharmaceutical Technology, National Research Centre, Dokki, CAiro, Egypt.
Mohamed S. El Ridy
Department of Pharmaceutical Technology, National Research Centre, Dokki, CAiro, Egypt.
Mona M. Abou Samra
Department of Pharmaceutical Technology, National Research Centre, Dokki, CAiro, Egypt.
Ghada E. A. Awad
Chemistry of Natural and Microbial Product Department, National Research Centre, Dokki, Cairo, Egypt.
Soheir S. Mansy
Electron Microscopy Research department Theodor bilharz Research Institute, Cairo, Egypt.
*Author to whom correspondence should be addressed.
Abstract
Aims: The objective of the current study was to formulate nystatin (Nyst) into nanostructured lipid carriers (NLCs) to enhance its antifungal activity.
Place and Duration of Study: Department of pharmaceutical technology, national research centre, Egypt, between mars 2011 to april 2013
Methodology: Nyst loaded NLCs (NYST-NLCs) were prepared by the hot homogenization and ultrasonication method followed by evaluation of its topical effect on the cutaneous candidiasis. The prepared Nyst-NLCs were characterized for entrapment efficiency, particle size, zeta potential, morphology (transmission electron microscopy), thermal characterisation (differential scanning calorimetry) and in vitro drug release. The study design involves the investigation of the effect of three independent variables namely liquid lipid type (Miglyol 812 and Squalene), liquid lipid concentration (30 and 50%) and Nyst concentration (0.125 and 0.25%). A stability study for 6 months was performed. A microbiological study was conducted in male rats infected with Candida albicans.
Results: NLC dispersions were spherical in shape with particle size ranging from 68.06±6.56 to 141.8±3.33 nm. The entrapment efficiencies ranged from 45.50±2.34 to 92.73±0.33% with zeta potential ranging from -26.2 to -39.2 mV. The stability studies done for 6 months indicated that Nyst-NLCs were stable for more than 6 monthes.the microbiological studies showed A least number of colonies forming units (cfu/ml) were recorded for the selected Nyst-NLCs compared to the drug solution and the Nystatin® cream present in the market.
Conclusion: It can be fulfilled from this work that NLCs represent promising carrier for topical delivery of Nyst offering good physical stability, high entrapment efficiency and controlled drug release. Nyst-NLCs are a good candidate for cutaneous treatment of fungal infection.
Keywords: Nanostructured lipid carriers, characterization, Nystatin, fungal infection, Candida albicans