Cyclodextrin-Based Supramolecular Multilayer Assemblies for the Design of Biological Optical Sensors Using Tilted Fiber Bragg Gratings

Sandrine Lépinay; Guillaume Laffont; Giséle Volet; Véronique Wintgens; Pierre Ferdinand; Marie Claude Millot; Benjamin Carbonnier

doi:10.4028/www.scientific.net/KEM.495.45

Paper Titles

Metamaterial Sensor Based on WGM
p.28

Fabrication of Carbon Nanotube/Low Density Polyethylene Composites for Strain Sensing
p.33

Nanostructures of Water Molecules in Iteratively Filtered Water
p.37

Metrological Performances of Smart Structures Based on Bragg Grating Sensors
p.41

Cyclodextrin-Based Supramolecular Multilayer Assemblies for the Design of Biological Optical Sensors Using Tilted Fiber Bragg Gratings
p.45

Cyclodextrin-Based Supramolecular Multilayer Assemblies for the Design of Chemical Optical Sensors Using Tilted Fiber Bragg Gratings
p.49

Metrological Performances of Fiber Bragg Grating Sensors and Comparison with Electrical Strain Gauges
p.53

Comparative Study in Magnetic Nanocomposites with PMMA and PS Matrices Made by Suspension Polymerization
p.58

New Ti-Alloy with Negative and Zero Thermal Expansion Coefficients
p.62

HomeKey Engineering MaterialsKey Engineering Materials Vol. 495Cyclodextrin-Based Supramolecular Multilayer...

Cyclodextrin-Based Supramolecular Multilayer Assemblies for the Design of Biological Optical Sensors Using Tilted Fiber Bragg Gratings

Abstract:

In this work, we demonstrate the possibility to use optical fiber incorporating photowritten tilted fiber Bragg gratings (TFBG) as optical detection system for the real time monitoring of interfacial adsorption events and biological recognition. For this purpose, immobilization of cyclodextrin polymers onto the surface of optical fiber was envisioned through the layer-by-layer self-assembly method with the aim of developing sensing layers with well-defined host properties. To develop a biological sensor, amphiphilic dextran, acting as intermediate layer between the polyelectrolyte multilayer assembly and the biological probe, was immobilized though inclusion complex formation. The dextran layer exhibit a dual functionality: (i) it prevents non-specific proteins adsorption and (ii) it allows covalent immobilization of anti-bovine serum albumine through activation of the hydroxyl groups with 1,1’-carbonyl diimidazole. To verify the feasibility of our strategy, fluorescence microscopy was applied to evidence the effective inclusion of fluorescent macromolecular – flurorescein labelled dextran bearing adamantane as side-grafts – species within the cyclodextrin cavities present onto the optical fiber interface and at the last layer to prove the grafting of anti bovin serum albumin onto the amphiphilic dextran by a capture of fluorescein bovin serum albumin by the antibody layer. In a further step, it was demonstrated that the elaboration of the multilayer assembly can be monitored in real time using the TFBG sensor.