Identification of Cell Apoptosis Morphology Induced by Oligosaccharides

Hong Lian Guo; Hai Yang Zhao; Yuan Yuan Sun

doi:10.4028/www.scientific.net/AMR.393-395.805

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HomeAdvanced Materials ResearchAdvanced Materials Research Vols. 393-395Identification of Cell Apoptosis Morphology...

Identification of Cell Apoptosis Morphology Induced by Oligosaccharides

Abstract:

Oligosaccharide as elicitor signal that induced the plant epidermal cells early apoptosis and death events were investigated In this paper.Onion epidermis was stained by typan blue and the rate of cells death is reached to 7.48% treated for 12h with 500ug / ml oligosaccharides, which were stained by fluorescence AO / EB treated with 50 ug/ml oligosaccharides and observed the cell morphology under fluorescence microscope,Some showed early apoptosis, such as the chromatin breakage, nuclear condensation, appearance of apoptotic bodies.

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Periodical:

Advanced Materials Research (Volumes 393-395)

Pages:

805-808

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.393-395.805

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Online since:

November 2011

Authors:

Hong Lian Guo, Hai Yang Zhao, Yuan Yuan Sun

Keywords:

Cell Apoptosis, Elicitor Signal, Fluorescent Stain, Oligosaccharide

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References

[1] Porat R, et al. Induction of chitinase and β-1, 3-endoglocanase Proteins by UV irradiation and wounding in grape fruit peel tissue . Phytoparasitica, 1999, 27(3): 233-238.

DOI: 10.1007/bf02981463

Google Scholar

[2] Yao HJ, Tian SP. Effects of a biocontrol agent and methyl jasmonate on post-harvest diseases of peach fruit and the possible mechanisms involved. J Appl Microbiol. 2005; 98(4): 941-50.

DOI: 10.1111/j.1365-2672.2004.02531.x

Google Scholar

[3] Mercier J, et al . Systemic and local responses associated with UV- and pathogen-induced resistance to Botrytis cinera in stored carrot. Phytopathology, 2000, 90(9): 981-986.

DOI: 10.1094/phyto.2000.90.9.981

Google Scholar

[4] Huang M, Khor E, Lim LY. Uptake and cytotoxicity of chitosan molecules and nanoparticles: effects of molecular weight and degree of deacetylation [J]. PharmRes, 2004, 21( 2) : 344- 353.

DOI: 10.1023/b:pham.0000016249.52831.a5

Google Scholar

[5] A. Zuppini, B. Baldan, R. Millioni, F. Favaron, L. Navazio, P. Mariani, Chitosan induces Ca2+mediated programmed cell death insoybean cells, New Phytol. 161 (2003) 557–568.

DOI: 10.1046/j.1469-8137.2003.00969.x

Google Scholar

[6] J.C. Cabrera, J. Messiaen, P. Cambier, P. Van Cutsem, Size, acetylation and concentration of chito-oligosaccharide elicitors determine the switch from defence involving PAL activation to cell death and water peroxide production in Arabidopsis cell suspensions, Physiol. Plantarum 127 (2006).

DOI: 10.1111/j.1399-3054.2006.00677.x

Google Scholar

[7] Hammerman PS, Fox CJ, Thompson CB. Beginnings of a signal-transduction pathway for bioenergetic control of cell survival [J]. Trends Biochem Scie, 2004, 29: 586-592.

DOI: 10.1016/j.tibs.2004.09.008

Google Scholar

[8] Wenxia Wang, Oligochitosan induces cell death and hydrogen peroxide accumulation in tobacco suspension cells. Pesticide Biochemistry and Physiology 90 (2008) 106–113.

DOI: 10.1016/j.pestbp.2007.10.003

Google Scholar