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Rapid Quantitative Detection of E.coli O157:H7 by a Impedance Immunosensor Based on Four-Wire Interdigitated Microelectrodes

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Abstract:

Rapid-response biosensing systems are necessary to counteract threats due to high-consequence pathogens. A disposable immunosensor suitable for quantitative determination of E.coli O157:H7 in pure cultures was investigated by electrochemical impedance spectroscopy (EIS). Electric responses of this biosensor for different cell concentrations at multi-frequencies were explored utilizing disposable screen-printed silver four-wire interdigitated microelectrodes (IMEs). Additionally, the best response frequency for the detection was studied, and the relationship between the impedance at this frequency and the concentrations of E.coli O157:H7 was established. The results showed that the impedance biosensor showed linearity from 1.15×103 CFU/mL to 1.15×106 CFU/mL at 100Hz, which yielded the model coefficient to 0.951.

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Periodical:

Applied Mechanics and Materials (Volumes 738-739)

Pages:

111-115

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.738-739.111

Citation:

Cite this paper

Online since:

March 2015

Authors:

Hai Yun Wu*, Xiao Ying Guo, Ya Ping Yu, Yue Ming Zuo, Yong Wei, Yuan Liu, Jin Xian Ma

Keywords:

E.coli O157:H7, Immunosensor, Impendance, Microelectrode, Quantitative Determination

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* - Corresponding Author

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