Paper Titles

Micro Circular Tube Flow Mathematical Model with the Effect of Van der Waals Force
p.3975

The Optimal Selection of the Conditions on Testing Starting Pressure Gradient for Tight Sandstone Oil Fields
p.3982

Determination of Hydrogeological Parameters of Aquifer Using the Air Compressor Pumping
p.3989

Effect of Salinity and Temperature on Swelling Behavior of Nano/Micro Polymer Particle
p.3993

Heavy-Oil Anaerobic Degradation and Methanogenic Characterization
p.3998

Numerical Simulation of the Buried Hill Reservoir in Bohai Bay
p.4003

The Research on Main Controlling Factors of Edge Water Invasion in Heavy Oil Thermal Recovery
p.4009

A Study on Factors Influencing the Effect of CO₂Flooding in F48 Block
p.4015

The Research on Distribution of the Remaining Oil in Heavy Oil Reservoir of BQ57
p.4022

HomeApplied Mechanics and MaterialsApplied Mechanics and Materials Vols. 448-453Heavy-Oil Anaerobic Degradation and Methanogenic...

Heavy-Oil Anaerobic Degradation and Methanogenic Characterization

Article Preview

Abstract:

Heavy oil Anaerobic degradation by microbial had important theoretical significance and application value of reservoir residual oil biological gasification. The microbial consortium SLY-1 which was enriched from heavy oil reservoir in Shengli oilfield had been cultured at 55°C. DGGE showed that there were two isolated strains which were Anaeromyxobacter sp. and Anaerobaculum sp. respectively and three uncultured bacteria included in SLY-1. After 380d’s culture, SLY-1 had been produced 1006 μmol of methane accounting for 95.2% and 4.8% of other gases (butane, pentane, 2,2-two methyl butane hydrocarbon, 2-cyclopentane etc.). The rate of heavy oil degradation by microbial consortium SLY-1 reached 30.6%. The high viscous microbial consortium could lay the foundation for the residual low-grade heavy oil gasification.

You might also be interested in these eBooks

Renewable Energy and Environmental Technology

Info:

Periodical:

Applied Mechanics and Materials (Volumes 448-453)

Pages:

3998-4002

DOI:

https://doi.org/10.4028/www.scientific.net/AMM.448-453.3998

Citation:

Cite this paper

Online since:

October 2013

Authors:

Gui Zhou Gu, Qiang Zhang, Mao Dong Zhang, Dong Feng Zhao*

Keywords:

Anaerobic Degradation, Degrading Characteristics, Heavy Oil Degradation, Methanogen, Microbial Consortium

Export:

RIS, BibTeX

Price:

Permissions:

Request Permissions

* - Corresponding Author

[1] A. Wentzel, T.E. Ellingsen, H.K. Kotlar, S.B. Zotchev and M. Throne-Holst, Bacterial metabolism of long-chain n-alkanes, Applied Microbiology and Biotechnology. 76 (2007) 1209-1221.

DOI: 10.1007/s00253-007-1119-1

[2] V. Grossi, C. Cravo-Laureau, R. Guyoneaud, A. Ranchou-Peyruse and A. Hirschler-Réa, Metabolism of n-alkanes and n-alkenes by anaerobic bacteria: A summary, Organic Geochemistry. 39 (2008) 1197-1203.

DOI: 10.1016/j.orggeochem.2008.02.010

[3] K. Zengler, H.H. Richnow, M.R. Rossello, W. Michaelis and F. Widdel, Methane formation from long-chain alkanes by anaerobic microorganisms, Nature. 401 (1999) 266-269.

DOI: 10.1038/45777

[4] R.T. Anderson, D.R. Lovley, Hexadecane decay by methanogenesis, Nature. 404 (2000) 722-723.

DOI: 10.1038/35008145

[5] D.M. Jones, I.M. Head, N.D. Gray, J. J. Adams, A. K. Rowan, C. M. Aitken, B. Bennett, H. Huang, A. Brown, B. F. J. Bowler, T. Oldenburg, M. Erdmann and S. R. Larter, Crude-oil biodegradation via methanogenesis in subsurface petroleum reservoirs, Nature. 451 (2008).

DOI: 10.1038/nature06484

[6] H. Li, S.Z. Yang, B.Z. Mu, Z.F. Rong and J. Zhang, Molecular analysis of bacterial community structure in a continental high-temperature and water-flooded petroleum reservoir, FEMS Microbiology Letters. 257 (2006) 92-98.

DOI: 10.1111/j.1574-6968.2006.00149.x

[7] A.V. Milkov, Secondary microbial origin of gas in giant cenomanian pools of western siberia, Abstract for AAPG Annual Convention and Exhibition, Denver, Colorado. 6 (2009) 7-10.

[8] M.T. Bao, B.Z. Mu, X.L. Wang, Analysis of metabolites of microorganisms used for oil recovery, Oilfield Chemistry. 19 (2002) 187-191.

[9] S. Chen, H. Li, S.Z. Yang and B.Z. Mu, Detection of diversity of hydrocarbon-degrading bacteria in oilfield production fluid by PCR-DGGE, Journal of microbiology. 30(2010) 1-6.

[10] B. Hendrickx, W. Dejonghe, F. Faber, W. Boënne, L. Bastiaens, W. Verstraete, E.M. Top and D. Springael, PCR-DGGE method to assess the diversity of BTEX mono-oxygenase genes at contaminated sites, FEMS Microbiology Ecology. 55 (2006) 262-273.

DOI: 10.1111/j.1574-6941.2005.00018.x

[11] C. Hallmann, L. Schwark, K. Grice, Community dynamics of anaerobic bacteria in deep petroleum reservoirs, Nature Geoscience. (2008) 588-591.

DOI: 10.1038/ngeo260

[12] V.D. Pham, L.L. Hnatow, S. Zhang, R.D. Fallon, S.C. Jackson, J.F. Tomb, E.F. DeLong and S.J. Keeler, Characterizing microbial diversity in production water from an Alaskan mesothermic petroleum reservoir with two independent molecular methods, Environmental Microbiology. 11 (2009).

DOI: 10.1111/j.1462-2920.2008.01751.x

[13] W.E. Balch and R.S. Wolfe, New approach to the Cultivation of methanogenic bacteria: 2-mercaptoethanesulfonic acid(HS-CoM)-dependent growth of Methanobacterium ruminantium in a pressureized atmosphere, Applied and Environmental Microbiology. 32 (1976).

DOI: 10.1128/aem.32.6.781-791.1976

[14] O. Kniemeyer, F. Musat , S.M. Sievert, K. Knittel, H. Wilkes, M. Blumenberg, W. Michaelis, A. Classen, C. Bolm, S.B. Joye and F. Widdel, Anaerobic oxidation of short-chain hydrocarbons by marine sulphate reducing bacteria, Nature. 449 (2007).

DOI: 10.1038/nature06200

[15] G. Muyzer, DGGE/TGGE a method for identifying genes from natural ecosystems, Current Opinion in Microbiology. 2 (1999) 317-322.

DOI: 10.1016/s1369-5274(99)80055-1

[16] Z. Li, C.C. Zhao, Y.B. Zhang and D.F. Zhao, Bioremediation of 16 EPA-PAHs combined contaminated-soil with microbial consortium, Journal of China University of Petroleum. 36 (2012) 175-181.