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Layer-by-layer adsorption of biopolyelectrolytes as a universal approach to fabrication of protein-loaded microparticles

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Moscow University Chemistry Bulletin Aims and scope

Abstract

This study was aimed at examination of microparticles formed via the layer-by-layer adsorption of dextran sulfate and chitosan onto the insoluble complexes of various proteins with polyanions. Microparticles with all tested proteins were stable at pH values of 1–5. At pH > 6 the mucoadhesivity of the microparticles changed and the encapsulated proteins were released. Microparticles were able to protect the proteins from proteases. Proteinous protease inhibitors encapsulated as well (2–3%) completely prevented protein proteolysis. The pharmacological effect of microencapsulated insulin was studied in vivo using the model of chronic diabetes in rats, which were treated by oral administration.

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Authors and Affiliations

  1. Department of Chemistry, Moscow State University, Moscow, 119991, Russia

    N. G. Balabushevich, M. A. Pechenkin, A. V. Lopes de Gerenyu, I. N. Zorov & N. I. Larionova

  2. Scientific Research Institute of Physico-Chemical Medicine, Moscow, Russia

    E. V. Mikhalchik

Authors
  1. N. G. Balabushevich
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  2. M. A. Pechenkin
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  3. A. V. Lopes de Gerenyu
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  4. I. N. Zorov
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  5. E. V. Mikhalchik
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  6. N. I. Larionova
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Corresponding author

Correspondence to N. G. Balabushevich.

Additional information

Original Russian Text © N.G. Balabushevich, M.A. Pechenkin, A.V. Lopes de Gerenyu, I.N. Zorov, E.V. Mikhalchik, N.I. Larionova, 2014, published in Vestnik Moskovskogo Universiteta. Khimiya, 2014, No. 3, pp. 158–166.

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Balabushevich, N.G., Pechenkin, M.A., Lopes de Gerenyu, A.V. et al. Layer-by-layer adsorption of biopolyelectrolytes as a universal approach to fabrication of protein-loaded microparticles. Moscow Univ. Chem. Bull. 69, 117–124 (2014). https://doi.org/10.3103/S002713141403002X

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  • DOI: https://doi.org/10.3103/S002713141403002X

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