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Improved DPPH determination for antioxidant activity spectrophotometric assay

  • M. Szabo EMAIL logo , C. Idiţoiu , D. Chambre and A. Lupea
From the journal Chemical Papers

Abstract

An improved procedure for determination of the residual DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical concentration was proposed taking into account the absorbance of both DPPH free radicals and DPPH nonradical (1,1-diphenyl-2-picrylhydrazine) stable form. The calculated residual DPPH free radical concentrations were compared with those obtained from a calibration curve and variation coefficients below 10 % were found.

[1] Pourmorad, F., Hosseinimehr, S. J., and Shahabimajd, N., Afr. J. Biotechnol. 5, 1142 (2006). Search in Google Scholar

[2] Sun, Y. M., Wang, R. X., Yuan, S. L., Lin, X. J., and Liu, C. B., Chin. J. Chem. 22, 827 (2004). http://dx.doi.org/10.1002/cjoc.2004022081210.1002/cjoc.20040220812Search in Google Scholar

[3] Cook, N. C. and Samman, S., J. Nutr. Biochem. 7, 66 (1996). http://dx.doi.org/10.1016/0955-2863(95)00168-910.1016/0955-2863(95)00168-9Search in Google Scholar

[4] Halliwell, B., Aeschbach, R., Loliger, J., and Aruoma, O. I., Food Chem. Toxicol. 33, 601 (1995). http://dx.doi.org/10.1016/0278-6915(95)00024-V10.1016/0278-6915(95)00024-VSearch in Google Scholar

[5] Aruoma, O. I., Free Radical Biol. Med. 20, 675 (1996). http://dx.doi.org/10.1016/0891-5849(95)02110-810.1016/0891-5849(95)02110-8Search in Google Scholar

[6] Huang, D. J., Chen, H. J., Lin, C. D., and Lin, Y. H., Bot. Bull. Acad. Sin. 46, 99 (2005). Search in Google Scholar

[7] Halliwell, B., Biochem. Pharmacol. 49, 1341 (1995). http://dx.doi.org/10.1016/0006-2952(95)00088-H10.1016/0006-2952(95)00088-HSearch in Google Scholar

[8] Vaya, J. and Aviram, M., Curr. Med. Chem.-Immunol. Endocr. Metab. Agents 1, 99 (2001). http://dx.doi.org/10.2174/156801301335916810.2174/1568013013359168Search in Google Scholar

[9] Amić, D., Davidović, D., Bešlo, D., and Trinajstić, N., Croat. Chem. Acta 76, 55 (2003). Search in Google Scholar

[10] Middleton, E., Kandaswami, C., and Theoharides, C., Pharmacol. Rev. 52, 673 (2000). Search in Google Scholar

[11] Benzie, I. F. F. and Strain, J. J., Anal. Biochem. 239, 70 (1996). http://dx.doi.org/10.1006/abio.1996.029210.1006/abio.1996.0292Search in Google Scholar

[12] Dapkevicius, A., Venskutonis, R., Beek, T. A., and Linssen, J. P. H., J. Sci. Food Agric. 77, 140 (1998). http://dx.doi.org/10.1002/(SICI)1097-0010(199805)77:1<140::AID-JSFA18>3.0.CO;2-K10.1002/(SICI)1097-0010(199805)77:1<140::AID-JSFA18>3.0.CO;2-KSearch in Google Scholar

[13] Dawidowicz, A. L., Wianowska, D., and Baraniak, B., LWT-Food Sci. Technol. 39, 308 (2006). http://dx.doi.org/10.1016/j.lwt.2005.01.00510.1016/j.lwt.2005.01.005Search in Google Scholar

[14] Silva, F. A. M., Borges, M. F. M., and Ferreira, M. A., ECSOC-2, [dp259], 1998; http://www.mdpi.org/ecsoc/ Search in Google Scholar

[15] Proestos, C., Boziaris, I. S., Nychas, G. J. E., and Komaitis, M., Food Chem. 95, 664 (2006). http://dx.doi.org/10.1016/j.foodchem.2005.01.04910.1016/j.foodchem.2005.01.049Search in Google Scholar

[16] Li, Y. F., Guo, C. J., Yang, J. J., Wei, J. Y., Xu, J., and Cheng, S., Food Chem. 96, 254 (2006). http://dx.doi.org/10.1016/j.foodchem.2005.02.03310.1016/j.foodchem.2005.02.033Search in Google Scholar

[17] Tepe, B., Sokmen, M., Akpulat, H. A., and Sokmen, A., Food Chem. 95, 200 (2006). http://dx.doi.org/10.1016/j.foodchem.2004.12.03110.1016/j.foodchem.2004.12.031Search in Google Scholar

[18] Katalinic, V., Milos, M., Kulisic, T., and Jukic, M., Food Chem. 94, 550 (2006). http://dx.doi.org/10.1016/j.foodchem.2004.12.00410.1016/j.foodchem.2004.12.004Search in Google Scholar

[19] Gong, Y., Toivonen, P. M. A., Lau, O. L., and Wiersma, P. A., Bot. Bull. Acad. Sin. 42, 259 (2001). Search in Google Scholar

[20] Thippeswamy, N. B. and Naidu, K. A., Eur. Food Res. Technol. 220, 472 (2005). http://dx.doi.org/10.1007/s00217-004-1087-y10.1007/s00217-004-1087-ySearch in Google Scholar

[21] Molyneux, P., Songklanakarin J. Sci. Technol. 26, 211 (2004). Search in Google Scholar

[22] Reschiglian, P., Zattoni, A., Melucci, D., Locatelli, C., Torsi, G., and Pandalai, S. G., Recent Research Developments in Applied Spectroscopy, Vol. 3, p. 61. Research Signpost, Trivandrum, INDE, 2000. Search in Google Scholar

[23] Scapim, C. A., Carvalho, C. G. P., and Cruz, C. D., Pesquisa Agr. Bras. 30, 683 (1995). Search in Google Scholar

[24] Blois, M. S., Nature 181, 1199 (1958). http://dx.doi.org/10.1038/1811199a010.1038/1811199a0Search in Google Scholar

[25] Gomez-Alonso, S., Fregapane, G., Salvador, M. D., and Gordon, M. H., J. Agric. Food Chem. 51, 667 (2003). http://dx.doi.org/10.1021/jf025932w10.1021/jf025932wSearch in Google Scholar

[26] Lebeau, J., Furman, C., Bernier, J. L., Duriez, P., Teissier, E., and Cotelle, N., Free Radical Biol. Med. 29, 900 (2000). http://dx.doi.org/10.1016/S0891-5849(00)00390-710.1016/S0891-5849(00)00390-7Search in Google Scholar

[27] Yepez, B., Espinosa, M., Lopez, S., and Bolanos, G., Fluid Phase Equilib. 194, 879 (2002). http://dx.doi.org/10.1016/S0378-3812(01)00707-510.1016/S0378-3812(01)00707-5Search in Google Scholar

[28] Bondet, V., Brand-Williams, W., and Berset, C., Food Sci. Technol. 30, 609 (1997). Search in Google Scholar

[29] Schwarz, K., Bertelsen, G., Nissen, L. R., Gardner, P. T., Heinonen, M. I., Hopia, A., Huynh-Ba, T., Lambelet, P., McPhail, D., Skibsted, L. H., and Tijburg, L., Eur. Food Res. Technol. 212, 319 (2001). http://dx.doi.org/10.1007/s00217000025610.1007/s002170000256Search in Google Scholar

[30] Mosquera, O. M. M., Nino, J. O., Correa, Y. M., and Buitrago-Bejarano, D. C., Sci. Tech. 27, 231 (2005). Search in Google Scholar

[31] Lee, K. W., Kim, Y. J., Lee, H. J., and Lee, C. Y., J. Agric. Food Chem. 51, 7292 (2003). http://dx.doi.org/10.1021/jf034438510.1021/jf0344385Search in Google Scholar PubMed

[32] Leitao, G. G., Laitao, S. G., and Vilegas, W., Z. Naturforsch., C: Biosci. 57, 1051 (2002). Search in Google Scholar

[33] Kim, J.-K., Noh, J. H., Lee, S., Choi, J. S., Suh, H., Chung, H. Y., Song, Y.-O., and Choi, W. C., Bull. Korean Chem. Soc. 23, 661 (2002). http://dx.doi.org/10.5012/bkcs.2002.23.5.66110.5012/bkcs.2002.23.5.661Search in Google Scholar

[34] Szabo, M. R., Chambre, D., and Idioiu, C., Scientific and Technical Bulletin of “Aurel Vlaicu” University, Series: Chemistry, Food Science, and Engineering 11, 122 (2006): www.uav.ro Search in Google Scholar

Published Online: 2007-6-1
Published in Print: 2007-6-1

© 2007 Institute of Chemistry, Slovak Academy of Sciences

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