Abstract
An electrochemical biosensor was developed based on formaldehyde dehydrogenase immobilized with Nafion membrane for determination of formaldehyde in fish. The enzyme was immobilized through the entrapment technique and measured based on the reduction of &bgr;-nicotinamide adenine dinucleotide. The response time of the formaldehyde biosensor was <1 min, with an optimum pH of 8. The optimum enzyme loading and NAD+ concentrations were found at 30 mg/mL and 0.5 mM, respectively. Using the formaldehyde biosensor, a linear response of formaldehyde showed a range of 0.1 to 10 ppm and a detection limit of 0.016 ppm. In application of Nash method, the samples were stored at 4°C ± 1 for 10 days. With the two combined methods, a linear correlation coefficient with R2 = 0.9982 (y = 0.956x - 0.014) was found. The developed formaldehyde biosensor showed a good reproducibility, long storage stability (more than 6 months stored at 4°C), and also effective monitoring of formaldehyde level in Indian mackerel (Rastrelliger kanagurta) fish.
Keywords: Electrochemical biosensor, Formaldehyde dehydrogenase, Formaldehyde, Nafion, Indian Mackerel.
Current Analytical Chemistry
Title:Electrochemical Biosensor Immobilization of Formaldehyde Dehydrogenase with Nafion for Determination of Formaldehyde from Indian Mackerel (Rastrelliger kanagurta) Fish
Volume: 8 Issue: 4
Author(s): NurIndang Marzuki, Fatimah Abu Bakar, Abu Bakar Salleh, Lee Yook Heng, Nor Azah Yusof and Shafiquzzaman Siddiquee
Affiliation:
Keywords: Electrochemical biosensor, Formaldehyde dehydrogenase, Formaldehyde, Nafion, Indian Mackerel.
Abstract: An electrochemical biosensor was developed based on formaldehyde dehydrogenase immobilized with Nafion membrane for determination of formaldehyde in fish. The enzyme was immobilized through the entrapment technique and measured based on the reduction of &bgr;-nicotinamide adenine dinucleotide. The response time of the formaldehyde biosensor was <1 min, with an optimum pH of 8. The optimum enzyme loading and NAD+ concentrations were found at 30 mg/mL and 0.5 mM, respectively. Using the formaldehyde biosensor, a linear response of formaldehyde showed a range of 0.1 to 10 ppm and a detection limit of 0.016 ppm. In application of Nash method, the samples were stored at 4°C ± 1 for 10 days. With the two combined methods, a linear correlation coefficient with R2 = 0.9982 (y = 0.956x - 0.014) was found. The developed formaldehyde biosensor showed a good reproducibility, long storage stability (more than 6 months stored at 4°C), and also effective monitoring of formaldehyde level in Indian mackerel (Rastrelliger kanagurta) fish.
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Cite this article as:
Marzuki NurIndang, Bakar Abu Fatimah, Salleh Bakar Abu, Heng Yook Lee, Yusof Azah Nor and Siddiquee Shafiquzzaman, Electrochemical Biosensor Immobilization of Formaldehyde Dehydrogenase with Nafion for Determination of Formaldehyde from Indian Mackerel (Rastrelliger kanagurta) Fish, Current Analytical Chemistry 2012; 8 (4) . https://dx.doi.org/10.2174/157341112803216843
DOI https://dx.doi.org/10.2174/157341112803216843 |
Print ISSN 1573-4110 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-6727 |
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