Stability of Asp^B28 Insulin Exposed to Modified and Unmodified Polypropylene

Title:Stability of Asp^B28 Insulin Exposed to Modified and Unmodified Polypropylene

Volume: 22 Issue: 7

Author(s): Charlotte J. Fristrup, Katja Jankova, Rüya Eskimergen, Jens T. Bukrinsky and Soren Hvilsted

Affiliation:

Keywords: Chemical stability, hydrophilic coating, insulin fibrillation, physical stability, surface initiated polymerization.

Abstract: Polypropylene (PP) plates have been modified with two different hydrophilic polymeric materials, poly(N,N-dimethylacrylamide) (poly(DMAAm)) and poly(poly(ethylene glycol)methacrylate) (poly(PEGMA)) in order to reduce insulin adsorption when the plates were exposed to insulin aspart (Asp^B28 insulin). The influence of surface modification on the chemical and physical stability of Asp^B28 insulin was evaluated by two chromatographic methods, size exclusion chromatography (SEC) and reverse phase high pressure liquid chromatography (RP-HPLC) and the Thioflavin T assay. A clear difference in the stability of Asp^B28 insulin was observed between the three tested surfaces. PP coated with poly(DMAAm) resulted in a poor chemical stability and a significantly improved physical stability compared with unmodified PP. In addition to this a lower phenol concentration was observed for the poly(DMAAm) coating. The results from the poly(PEGMA) coating looked very promising with respect to the stability of Asp^B28 insulin in comparison with the data from unmodified PP and the poly(DMAAm) coating. Two hydrophilic coatings have been tested and surprisingly a difference in Asp^B28 insulin stability was observed. Therefore, Asp^B28 insulin adsorption and stability will be influenced by more than the hydrophilicity of the surface.

Cite this article as:

Fristrup J. Charlotte, Jankova Katja, Eskimergen Rüya, Bukrinsky T. Jens and Hvilsted Soren, Stability of Asp^B28 Insulin Exposed to Modified and Unmodified Polypropylene, Protein & Peptide Letters 2015; 22 (7) . https://dx.doi.org/10.2174/0929866522666150526154007