Skip to main content
SpringerLink
Log in

Suppression of Non-specific Adsorption Using Densified Tri(ethylene glycol) alkanethiols: Monolayer Characteristics Evaluated by Electrochemical Measurements

  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

Tri(ethylene glycol) terminated short alkylchain thiols (TEGCnSHs) offer good potential for constructing ultra-thin protein-resistant monolayers because they have an alkylchain for forming a densely packed monolayer and a flexible-hydrophilic oligo ethylene glycol arm for avoiding non-specific adsorption. Hybrid monolayers consisting of TEGCnSH and a maltoside ligand (MalC12SH, for capturing lectin) were effective in detecting concanavalin A (Con A). This hybrid monolayer was more suitable for Con A detection than that modified with 100% ligands in terms of the detection limit and time. The anti-fouling properties, packing densities, interaction and homogeneity of TEGCnSH monolayers were confirmed in detail by surface plasmon resonance (SPR) measurements and electrochemical methods. SPR measurements revealed their excellent repellency to proteins and peptides of various sizes (MW 400 – 104000). The electrochemical results indicated that the lower defects in the TEGCnSH monolayers suppressed the permeation of small peptides. The stability, homogeneity and packing density of the TEGCnSH monolayers were gradually improved as their alkylchain length increased.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. “Hydrophilic Polymers, Performance with Environmental Acceptability”, ed. J. E. Glass, 1996, American Chemistry Society, Washington, D.C.

  2. K. Y. Suh, J. Seong, A. Khademhosseini, P. E. Laibinis, and R. Langer, Biomaterials, 2004, 25, 557.

    Article  CAS  PubMed  Google Scholar 

  3. T. Satomi, Y. Nagasaki, H. Kobayashi, H. Otsuka, and K. Kataoka, Langmuir, 2007, 23, 6698.

    Article  CAS  PubMed  Google Scholar 

  4. K. Yoshimoto, S. Matsumoto, R. Asakawa, K. Uchida, T. Ishii, and Y. Nagasaki, Chem. Lett., 2007, 36, 1444.

    Article  CAS  Google Scholar 

  5. B. Kannan, K. Castelino, F. F. Chen, and A. Majumdar, Biosens. Bioelectron., 2006, 21, 1960.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. M. Kyo, K. Usui-Aoki, and H. Koga, Anal. Chem., 2005, 77, 7115.

    Article  CAS  PubMed  Google Scholar 

  7. K. B. Lee, S. J. Park, C. A. Mirkin, J. C. Smith, and M. Mrksich, Science, 2002, 295, 1702.

    Article  CAS  PubMed  Google Scholar 

  8. M. Mrksich and G. M. Whitesides, “ACS Symposium #680: Poly(Ethylene Glycol): Chemistry and Biological Applications”, ed. J. M. Harris and S. Zalipsky, 1997, American Chemical Society, Washington, D.C., 361.

  9. C. Palegrosdemange, E. S. Simon, K. L. Prime, and G. M. Whitesides, J. Am. Chem. Soc., 1991, 113, 12.

    Article  CAS  Google Scholar 

  10. A. Fragoso, N. Laboria, D. Latta, and C. K. O’Sullivan, Anal. Chem., 2008, 80, 2556.

    Article  CAS  PubMed  Google Scholar 

  11. E. Ostuni, R. G. Chapman, M. N. Liang, G. Meluleni, G. Pier, and D. E. Ingber, Langmuir, 2001, 17, 6336.

    Article  CAS  Google Scholar 

  12. E. Ostuni, B. A. Grzybowski, M. Mrksich, C. S. Roberts, and G. M. Whitesides, Langmuir, 2003, 19, 1861.

    Article  CAS  Google Scholar 

  13. G. B. Sigal, M. Mrksich, and G. M. Whitesides, J. Am. Chem. Soc., 1998, 120, 3464.

    Article  CAS  Google Scholar 

  14. S. Herrwerth, W. Eck, S. Reinhardt, and M. Grunze, J. Am. Chem. Soc., 2003, 125, 9359.

    Article  CAS  PubMed  Google Scholar 

  15. K. L. Prime and G. M. Whitesides, J. Am. Chem. Soc., 1993, 115, 10714.

    Article  CAS  Google Scholar 

  16. Y. Sato, K. Yoshioka, M. Tanaka, T. Murakami, N. M. Ishida, and O. Niwa, Chem.commun., 2008, 4909.

  17. T. Murakami, R. Hirono, Y. Sato, and K. Furusawa, Carbohydr. Res., 2007, 342, 1009.

    Article  CAS  PubMed  Google Scholar 

  18. Y. Sato, T. Murakami, K. Yoshioka, and O. Niwa, Anal. Bioanal. Chem., 2008, 391, 2527.

    Article  CAS  PubMed  Google Scholar 

  19. J. Clavilier, R. Faure, G. Guinet, R. Durand, J. Electroanal. Chem., 1980, 107, 205.

    Article  CAS  Google Scholar 

  20. E. Stenberg, B. Persson, H. Roos, and C. Urbaniczky, J. Colloid Interface Sci., 1991, 143, 513.

    Article  CAS  Google Scholar 

  21. L. G. Fägerstam, Å. Frosell-Karlsson, R. Karlsson, B. Persson, and I. Rönnberg, J. Chromatogr., 1992, 597, 397.

    Article  PubMed  Google Scholar 

  22. C. Duncan, A. H. Haines, and D. A. Russell, Langmuir, 2003, 19, 7141.

    Article  Google Scholar 

  23. K. Uchida, H. Otsuka, M. Kaneko, K. Kataoka, and Y. Nagasaki, Anal. Chem., 2005, 77, 1075.

    Article  CAS  PubMed  Google Scholar 

  24. T. Satomi, Y. Nagasaki, H. Kobayashi, T. Tateishi, K. Kataoka, and H. Otsuka, J. Nanosci. Nanotechnol., 2007, 7, 2394.

    Article  CAS  PubMed  Google Scholar 

  25. M. M. Walczak, D. D. Popenoe, R. S. Deihammer, B. D. Lamp, C. Chung, and M. D. Porter, Langmuir, 1991, 7, 2687.

    Article  CAS  Google Scholar 

  26. H. O. Finklea, “Electroanalytical Chemistry”, ed. A. J. Bard and I. Rubinstein, 1996, Vol. 19, Chap. 2, Marcel Dekker, New York.

  27. S. Imabayashi, M. Iida, D. Hobara, Z. Q. Feng, K. Niki, and T. Kakiuchi, J. Electroanal. Chem., 1997, 428, 33.

    Article  CAS  Google Scholar 

  28. C. J. Zhong and M. D. Porter, J. Electroanal. Chem., 1997, 425, 147.

    Article  CAS  Google Scholar 

  29. T. Kakiuchi, H. Usui, D. Hobara, and M. Yamamoto, Langmuir, 2002, 18, 5231.

    Article  CAS  Google Scholar 

  30. C. A. Widrig, C. Chung, and M. D. Porter, J. Electroanal. Chem., 1991, 310, 335.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan

    Kyoko Yoshioka, Yukari Sato, Mutsuo Tanaka, Teiichi Murakami & Osamu Niwa

Authors
  1. Kyoko Yoshioka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yukari Sato
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mutsuo Tanaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Teiichi Murakami
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Osamu Niwa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yukari Sato.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yoshioka, K., Sato, Y., Tanaka, M. et al. Suppression of Non-specific Adsorption Using Densified Tri(ethylene glycol) alkanethiols: Monolayer Characteristics Evaluated by Electrochemical Measurements. ANAL. SCI. 26, 33–37 (2010). https://doi.org/10.2116/analsci.26.33

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.2116/analsci.26.33

Search

Navigation