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Enhancement of the Binding Ability of a Ligand for Nucleobase Recognition by Introducing a Methyl Group

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Abstract

The recognition ability of pteridine derivatives for nucleobases opposite an abasic (AP) site in an oligodeoxynucleotide (ODN) duplex is enhanced by using a propylene residue (Spacer-C3) as an AP site. The recognition ability is further enhanced both by attaching methyl groups to a fluorescent ligand and by measuring the fluorescence response at 5°C; 6.2 × 106 M-1 of the binding constant is attained between 2-amino-6,7-dimethyl-4-hydroxypteridine and guanine opposite the AP site in water.

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Authors and Affiliations

  1. Department of Chemistry, Graduate School of Science, Aoba-ku, Sendai 980-8578, Japan

    Qing Dai, Chun-Yan Xu, Yusuke Sato, Seiichi Nishizawa & Norio Teramae

  2. Bioengineering Laboratory, RIKEN (The Institute of Physical and Chemical Research), Hirosawa 2-1, Wako, Saitama, 351-0198, Japan

    Keitaro Yoshimoto

Authors
  1. Qing Dai
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  2. Chun-Yan Xu
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  3. Yusuke Sato
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  4. Keitaro Yoshimoto
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  5. Seiichi Nishizawa
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  6. Norio Teramae
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Corresponding author

Correspondence to Norio Teramae.

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Dai, Q., Xu, CY., Sato, Y. et al. Enhancement of the Binding Ability of a Ligand for Nucleobase Recognition by Introducing a Methyl Group. ANAL. SCI. 22, 201–203 (2006). https://doi.org/10.2116/analsci.22.201

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  • DOI: https://doi.org/10.2116/analsci.22.201

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