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Fluorometric Determination of DNA Using a New Ruthenium Complex Ru(bpy)2PIP(V) as a Nucleic Acid Probe

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Abstract

A new ligand, 2-phenyl(5-fluoro)imidazo[f]-l,10-phenanthroline (PIP(V)), and its coordination compounds, Ru(bpy)2PIP(V), were synthesized. The fluorescence spectrum of the interaction between Ru(bpy)2PIP(V) and DNA was studied, and a very strong fluorescence peak at a wavelength of 589 nm appeared. The optimum condition of analyzing DNA was decided. The method is simple, convenient and fast, and also has high sensitivity and good selectivity. It has been satisfactorily employed for determinations in synthesized samples.

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Authors and Affiliations

  1. Analysis and Testing Center Hubei University, Wuhan, 430062, China

    Gongwu Song, Ling Li, Liming Liu & Guangrong Fang

  2. College of Chemistry, Wuhan University, Wuhan, 430072, China

    Shaofang Lu, Zhike He & Yun’e Zeng

Authors
  1. Gongwu Song
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  2. Ling Li
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  3. Liming Liu
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  4. Guangrong Fang
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  5. Shaofang Lu
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  6. Zhike He
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  7. Yun’e Zeng
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Correspondence to Gongwu Song.

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Song, G., Li, L., Liu, L. et al. Fluorometric Determination of DNA Using a New Ruthenium Complex Ru(bpy)2PIP(V) as a Nucleic Acid Probe. ANAL. SCI. 18, 757–759 (2002). https://doi.org/10.2116/analsci.18.757

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  • DOI: https://doi.org/10.2116/analsci.18.757

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