ISHS Acta Horticulturae 535: First International Citrus Biotechnology Symposium
MAPPING THE CITRUS GENOME |
| Authors: | M.L. Roose, D. Feng, F.S. Cheng, R.I. Tayyar, C.T. Federici, R.S. Kupper |
| Keywords: | Poncirus trifoliata, linkage |
| DOI: | 10.17660/ActaHortic.2000.535.1 |
| Abstract:
The Citrus genome is being mapped to facilitate marker-assisted selection in the development of new cultivars, to clarify inheritance of important traits in various crosses, and to permit positional cloning of valuable genes. Several mapping efforts in Citrus have focused on Citrus x Poncirus hybrids because a high proportion of markers and rootstock traits will segregate in their progeny. A map based on segregation of 156-isozyme restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR), and inter-simple sequence repeat (ISSR) markers in a cross between two Citrus x Poncirus hybrids, Sacaton citrumelo and Troyer citrange, is described and compared with published maps. There has generally been little effort to interrelate maps developed in different laboratories because the maps share few common markers. The development and use of simple sequence repeat markers would facilitate map integration and allow marker-aided selection in a wider range of populations. Citrus maps have not been compared with those of other plants because citrus is distantly related to other crop plants and very few Citrus markers are derived from conserved gene sequences. The value of Citrus maps is increased by the recent development of bacterial artificial chromosome (BAC) libraries that permit cloning of mapped genes. | |
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