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Quantum Dots as Inorganic DNA-Binding Proteins

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Abstract

Semiconductor quantum dots of cadmium sulfide, CdS, are approximately the size of proteins and are photoluminescent in the red, yellow, or green, depending on surface preparation. This photoluminescence is very sensitive to the Nature and amount of adsorbates. We have found that DNAs with intrinsic curvature adsorb more strongly to the surface of 47 Å CdS quantum dots, as judged by concentration-dependent changes in photoluminescence. The binding constants we obtain are similar to those found for nonspecific protein-DNA interactions. The surface groups of the CdS substrate also influence DNA adsorption. Thus these protein-sized colloidal particles can be used in chemical sensing applications for curved or kinked DNA; DNA with unusual structures is thought to influence biological function such as transcription.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC, 29208, USA

    Catherine J. Murphy, Eric B. Brauns & Latha Gearheart

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  1. Catherine J. Murphy
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  2. Eric B. Brauns
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  3. Latha Gearheart
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Murphy, C.J., Brauns, E.B. & Gearheart, L. Quantum Dots as Inorganic DNA-Binding Proteins. MRS Online Proceedings Library 452, 597–600 (1996). https://doi.org/10.1557/PROC-452-597

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