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Nanomonitors: Electrical Immunoassays for Protein Biomarker Profiling

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Abstract

The objective of this research is to develop a “point-of-care” device for early disease diagnosis through protein biomarker characterization. Here we present label-free, high sensitivity detection of proteins with the use of electrical immunoassays that we call Nanomonitors. The basis of the detection principle lies in the formation of an electrical double layer and its perturbations caused by proteins trapped in a nanoporous alumina membrane over a microelectrode array platform. High sensitivity and rapid detection of two inflammatory biomarkers, C-reactive protein (CRP) and Myeloperoxidase (MPO) in pure and clinical samples through label-free electrical detection were achieved. The performance metrics achieved by this device makes it suitable as a “lab-on-a-chip” device for protein biomarker profiling and hence early disease diagnosis.

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Authors and Affiliations

  1. Electrical and Computer Engineering, Portland State University, 1900 SW Fourth Avenue Bldg, Wilsonville, OR, 97201, USA

    Manish Bothara

  2. Electrical and Computer Engineering, Portland State University, Portland, OR, 97201, USA

    Ravi K. Reddy & Shalini Prasad

  3. Veteran Affairs, Oregon Health and Science University, Portland, OR, 97201, USA

    Thomas Barrett

  4. Physics Department, Portland State University, Portland, OR, 97201, USA

    John Carruthers

Authors
  1. Manish Bothara
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  2. Ravi K. Reddy
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  3. Thomas Barrett
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  4. John Carruthers
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  5. Shalini Prasad
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Bothara, M., Reddy, R.K., Barrett, T. et al. Nanomonitors: Electrical Immunoassays for Protein Biomarker Profiling. MRS Online Proceedings Library 1106, 302 (2008). https://doi.org/10.1557/PROC-1106-PP03-02

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  • DOI: https://doi.org/10.1557/PROC-1106-PP03-02

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