Skip to main content
SpringerLink
Log in

Deconvolution of Self-encoded Polymer Beads in Random Microarrays for Antigen Biosensing by Raman Spectroscopy

  • Article
  • Published:
MRS Online Proceedings Library Aims and scope

Abstract

Random bead microarray in which each bead has been functionalized with different biomolecules are of great interest. The process by which one would identify the location of each bead is referred to as decoding. The decoding step has played a challenging role in microarray technologies due to the increase in complexity with the increase in array size. Here we report a novel, fast and reliable method of decoding randomly assembled arrays based on polymer beads with unique spectroscopic signatures. Beads were synthesized by dispersion polymerization of a family of styrene monomers and methacrylic acid to generate a spectroscopically encoded bead library. In addition to identifying the self-encoded beads through their unique spectrum in a tetraplex experiment, Raman spectroscopy was used to monitor antibody-antigen binding events on the barcoded beads. The simplicity, versatility and rapid analysis enabled by this self-encoded bead array platform technology demonstrates its potential in high-throughput biomolecular multiplex screening.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. S. Venkatasubbarao, Trends Biotechnol 22 (12), 630 (2004).

    Article  CAS  Google Scholar 

  2. K.L. Gunderson, F. Steemer, G. Lee et al., Nature Genetics 37, 549 (2005).

    Article  CAS  Google Scholar 

  3. Y. Xiaobo, X. Danke, and C. Quan, Proteomics 6, 5493 (2006).

    Article  Google Scholar 

  4. M.J. Heller, Annu. Rev. Biomed. Eng. 4, 129 (2002).

    Article  CAS  Google Scholar 

  5. H. Fenniri, S. Chun, L. H. Ding et al., J Am Chem Soc 125 (35), 10546 (2003).

    Article  CAS  Google Scholar 

  6. H. Fenniri, O. Terreau, S. K. Chun et al., J Combin Chem 8 (2), 192 (2006).

    CAS  Google Scholar 

  7. H. Fenniri, L. H. Ding, A. E. Ribbe et al., Journal of the American Chemical Society 123 (33), 8151 (2001).

    CAS  Google Scholar 

  8. J. P. Bravo-Vasquez, R. A. Alvarez-Puebla, and H. Fenniri, Sensors and Actuators B 125, 357 (2007).

    CAS  Google Scholar 

  9. S. Hu, J. A. Loo, and D. T. Wong, Proteomics 6 (23), 6326 (2006).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Institute for Nanotechnology, 11421 Saskatchewan Drive, T6G 2M9, Edmonton, Canada

    Juan P Bravo-Vasquez, Ramon A Alvarez-Puebla & Hicham Fenniri

  2. Steacie Institute for Molecular Sciences, 100 Sussex Drive, K1A 0R6, Ottawa, Canada

    David Blais & John P. Pezacki

  3. Department of Chemistry, University of Alberta, 11227 Saskatchewan Drive, T6G 2G2, Edmonton, Canada

    Ying Zhang, Jose Raez & Hicham Fenniri

  4. Department of Chemistry, University of Ottawa, 10 Marie-Curie, K1N 6N5, Ottawa, Canada

    John P. Pezacki

Authors
  1. Juan P Bravo-Vasquez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ramon A Alvarez-Puebla
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. David Blais
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ying Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jose Raez
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. John P. Pezacki
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Hicham Fenniri
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bravo-Vasquez, J.P., Alvarez-Puebla, R.A., Blais, D. et al. Deconvolution of Self-encoded Polymer Beads in Random Microarrays for Antigen Biosensing by Raman Spectroscopy. MRS Online Proceedings Library 1024, 410 (2007). https://doi.org/10.1557/PROC-1024-A04-10

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1557/PROC-1024-A04-10

Search

Navigation