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Entrapment of DFPase in titania coatings from a biomimetically derived method

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Abstract

Silicon oxide has been widely used to encapsulate biomolecules to preserve their activity in less than ideal environments. However, there are other inorganic oxides with inherent properties that would be advantageous in creating a multifunctional material. Titanium oxide exhibits properties that have applications in areas such as electronics, energy conversion, and decontamination. Herein is reported the formation of titania coatings fabricated on polymer beads using a biomimetic approach and characterized with scanning electron microscopy and energy dispersive x-ray spectroscopy. The approach involves the use of functionalized polymer beads, which initiate oxide formation from a water-soluble titanium complex. The method was used to encapsulate the enzyme diisopropylfluorophosphatase, in situ, within the oxide matrix under buffered aqueous conditions while retaining its enzymatic activity against diisopropylfluorophosphate. In addition, the biomimetically produced titania was shown to exhibit UV-assisted degradation activity against an ethidium bromide dye, upon liberation from the coating template.

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Acknowledgments

This research was funded by the Defense Threat Reduction Agency. The authors acknowledge Dr. John Walker for his assistance with the DFPase activity assay and Dr. Lian Li and Professor Jayant Kumar from the University of Massachusetts-Lowell for their assistance with x-ray diffraction of the titania-coated Sephadex beads.

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  1. US Army Natick Soldier Research, Development, and Engineering Center, Natick, Massachusetts, 01760, USA

    Shaun Filocamo, Robert Stote, David Ziegler & Heidi Gibson

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  1. Shaun Filocamo
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Correspondence to Shaun Filocamo.

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Filocamo, S., Stote, R., Ziegler, D. et al. Entrapment of DFPase in titania coatings from a biomimetically derived method. Journal of Materials Research 26, 1042–1051 (2011). https://doi.org/10.1557/jmr.2011.33

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