Immunohistochemistry of cytokeratins 7, 8, 17, 18, and 19, and GLUT-1 aids differentiation of desmoplastic malignant mesothelioma from fibrous pleuritis
Toshikatsu Horiuchi1, Sho Ogata1, Susumu Tominaga1, Sadayuki Hiroi1, Kunimitsu Kawahara2, Akira Hebisawa3, Isao Irei4, Ichiro Ito5, Toru Kameya5, Tohru Tsujimura6, Takashi Nakano7, Kuniaki Nakanishi8 and Toshiaki Kawai1
1Department of Pathology and Laboratory Medicine, National Defense Medical College, Tokorozawa,2Department of Pathology, Osaka Prefectural Medical Center for Respiratory and Allergic Diseases, Habikino, 3Department of Pathology, National Tokyo Hospital, Kiyose, 4Department of Pathology, Kawasaki Medical School, Kurashiki, 5Pathology Division, Shizuoka Cancer Center Hospital and Research Institute, Shizuoka, 6Division of Molecular Pathology, Department of Pathology and 7Division of Respiratory Medicine, Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya and 8Department of Laboratory Medicine, National Defense Medical College Hospital, Tokorozawa, Japan.
Offprint requests to: Sho Ogata MD, Department of Pathology and Laboratory Medicine, National Defense Medical College, Tokorozawa 359-8513, Japan. e-mail: sogata@ndmc.ac.jp
Summary. It is difficult to distinguish desmoplastic malignant mesothelioma (DMM) from fibrous pleuritis (FP). We investigated the utility of immunohisto-chemistry as a way of differentiating between DMM and FP. We examined 11 DMMs and 46 FPs with the aid of antibodies against 18 cytokeratin (CK) subtypes, calponin, caldesmon, desmin, and GLUT-1. The best sensitivity and specificity cut-off values in the receiver operating characteristic curves (ROC) for CKs 7, 8, 17, 18, and 19, and GLUT-1 were each above 60%. When cases with either DMM or FP were partitioned by the staining score associated with the best sensitivity and specificity cut-off values in ROC, the incidence of a positive expression for CKs 7, 8, 17, 18, and 19, and GLUT-1 was significantly higher in DMM than in FP. In conclusion, immunohistochemistry for CKs 7, 8, 17, 18, and 19, and GLUT-1 may be useful, alongside histological characteristics, for separating DMM from FP. Histol Histopathol 28, 663-670 (2013)
Key words: Immunohistochemistry, Desmoplastic malignant mesothelioma, Fibrous pleurisy, Cytokeratins, GLUT-1
DOI: 10.14670/HH-28.663