Summary
Currently, there is a growing interest in the study of natural products, especially as part of drug discovery programs. Secondary metabolites can be extracted from a variety of natural sources, including plants, microbes, marine animals, insects, and amphibia. This chapter focuses principally on laboratory-scale processes of initial and bulk extraction of natural products from plant and microbial sources. With regard to plant natural products, the steps required for the preparation of the material prior to extraction, including aspects concerning plant selection, collection, identification, drying, and grinding, are detailed. The various methods available for solvent extraction (maceration, percolation, Soxhlet extraction, pressurized solvent extraction, ultrasound-assisted solvent extraction, extraction under reflux, and steam distillation) are reviewed. Further focus is given on the factors that can influence the selection of a method and suitable solvent. Specific extraction protocols for certain classes of compounds are also discussed. Regarding microbial natural products, this chapter covers issues relating to the isolation of microorganisms and presents the extraction methods available for the recovery of metabolites from fermentation broths. Methods of minimizing compound degradation, artifact formation, extract contamination with external impurities, and enrichment of extracts with desired metabolites are also examined.
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References
Schliemann, W., Yizhong Cai, Y., Degenkolb, T., Schmidt, J., and Corke, H. (2001) Betalains of Celosia argentea. Phytochemistry 58, 159–165.
Brown, G. D., Liang, G.-Y., and Sy, L.-K. (2003) Terpenoids from the seeds of Artemisia annua. Phytochemistry 64, 303–323.
Takahashi, H., Hirata, S., Minami, H., and Fukuyama, Y. (2001) Triterpene and flavanone glycoside from Rhododendron simsii. Phytochemistry 56, 875–879.
Mohagheghzadeh, A., Schmidt, T. J., and Alfermann, A. W. (2002) Arylnaphthalene lignans from in vitro cultures of Linum austriacum. J. Nat. Prod. 65, 69–71.
Waksmundzka-Hajnos, M., Petruczynik, A., Dragan, A., Wianwska, D., Dawidowicz, A., and Sowa, I. (2004) Influence of the extraction mode on the yield of some furanocoumarins from Pastinaca sativa fruits. J. Chromatogr. B 800, 181–187.
Benthin, B., Danz, H., and Hamburger, M. (1999) Pressurized liquid extraction of medicinal plants. J. Chromatogr. A 837, 211–219.
Cottiglia, F., Dhanapal, B., Sticher, O., and Heilmann, J. (2004) New chromanone acids with antibacterial activity from Calophyllum brasiliens. J. Nat. Prod. 67, 537–541.
Lin, L.-C., Yang, L.-L., and Chou, C.-J. (2003) Cytotoxic naphthoquinones and plumbagic acid glucosides from Plumbago zeylanica. Phytochemistry 62, 619–622.
Akhtar, M. N., Atta-ur-Rahman, Choudhary, M. I., Sener, B., Erdogan, I., and Tsuda, Y. (2003) New class of steroidal alkaloids from Fritillaria imperialis. Phytochemistry 63, 115–122.
Zanolari, B., Guilet, D., Marston, A., Queiroz, E. F., Paulo, M. Q., and Hostettmann, K. (2003) Tropane alkaloids from the bark of Erythroxylum vacciniifolium. J. Nat. Prod. 66, 497–502.
Zhang, X., Yea, W., Zhaoa, S., and Che, C.-T. (2004) Isoquinoline and isoindole alkaloids from Menispermum dauricum. Phytochemistry 65, 929–932.
Toki, K., Saito, N., Shigihara, A., and Honda, T. (2001) Anthocyanins from the scarlet flowers of Anemone coronaria. Phytochemistry 56, 711–715.
Kiehlmann, E. and Li, E. P. M. (1995) Isomerisation of dihydroquercetin. J. Nat. Prod. 58, 450–455.
Sulaiman, M., Martin, M. T., Pais, M., Hadi, H. A., and Awang, K. (1998) Desmosine, an artefact alkaloid from Desmos dumosus. Phytochemistry 49, 2191–2192.
Salim, A. A., Garson, M. J., and Craik, D. J. (2004) New Alkaloids from Pandanus amaryllifolius. J. Nat. Prod. 67, 54–57.
Funayama S., Ishibashi M., Ankaru Y., et al. (1989) Novel cytocidal antibiotics, glucopiericidinols A1 and A2. Taxonomy, fermentation, isolation, structure elucidation and biological characteristics. J. Antibiot. 42,1734–1740.
Cao S., Lee A. S. Y., Huang Y., et al. (2002) Agonodepsides A and B: two new depsides from a filamentous fungus F7524. J. Nat. Prod. 65, 1037–1038.
Chinworrungsee, M., Kittakoop, P., Isaka, M., Maithip, P., Supothina, S., and Thebtaranonth, Y. (2004) Isolation and structure elucidation of a novel antimalarial macrocyclic polylactone, menisporopsin A, from the fungus Menisporopsis theobromae. J. Nat. Prod. 67, 689–692.
Machida K., Trifonov I. S., Ayer W. A., et al. (2001) 3(2H)-Benzofuranones and chromanes from liquid cultures of the mycoparasitic fungus Coniothyrium minitans. Phytochemistry 58, 173–177.
Liu, Z., Jensen, P. R., and Fenical, W. (2003) A cyclic carbonate and related polyketides from a marine-derived fungus of the genus Phoma. Phytochemistry 64, 571–574.
Shiono, Y., Matsuzaka, R., Wakamatsu, H., Muneta, K., Murayama, T., and Ikeda, M. (2004) Fascicularones A and B from a mycelial culture of Naematoloma fasciculare. Phytochemistry 65, 491–496.
Yun, B.-S., Lee, I.-K., Cho, Y., Cho, S.-M., and Yoo, I.-D. (2002) New Tricyclic Sesquiterpenes from the fermentation Broth of Stereum hirsutum. J. Nat. Prod. 65, 786–788.
Gerth, K., Bedorf, N., Irschik, H., Hofle, G., and Reichenbach, H. (1994) The soraphens: a family of novel antifungal compounds from Sorangium cellulosum (Myxobacteria) I. Soraphen A1α: fermentation, isolation, biological properties. J. Antibiot. 47, 23–31.
Demain, A. L. (1999) Pharmaceutically active secondary metabolites of microorganisms. Appl. Microbiol. Biotechnol. 52, 455–463.
Phillipson, J. D. and Bisset, N. G. (1972) Quaternisation and oxidation of strychnine and brucine during plant extraction. Phytochemistry 11, 2547–2553.
Lavie, D., Bessalle, R., Pestchanker, M. J., Gottlieb, H. E., Frolow, F., and Giordano, O. S. (1987) Trechonolide A, a new withanolide type from Trechonaetes laciniata. Phytochemistry 26, 1791–1795.
Banthorpe, D. V. (1991) Classification of terpenoids and general procedures for their characterisation, in Methods in Plant Biochemistry, vol. 7 (Dey, P. M. and Harborne, J. B., eds.) Academic, New York.
Suggested Reading
Bruneton J. (1995) Pharmacognosy, Phytochemistry, Medicinal Plants. Springer-Verlag, Berlin.
Evans W. C. (2002) Trease and Evans’ Pharmacognosy, 15 ed, Bailliere Tindall: London.
Heinrich M., Barnes J., Gibbons S., and Williamson E. M. (2004) Fundamentals of Pharmacognosy and Phytotherapy. Churchill Livingstone, Edinburgh.
Kaufman, P. B., Cseke, L. J., Warber, S., Duje, J. A., and Brielman, H. L. (1999) Natural Products From Plants, CRC Press, Boca Raton
Lancini G. and Lorenzetti R. (1993) Biotechnology of Antibiotics and Other Bioactive Microbial Metabolites. Plenum, New York.
List P. H. and Schmit P. C. (1989) Phytopharmaceutical Technology. Heyden & Son Ltd, London.
Williamson E. M., Okpako D. T., and Evans F. J. (1996) Selection, preparation and pharmacological evaluation of plant material, in Pharmacological Methods in Phytotherapy Research, vol. 1. John Wiley and Sons, Chichester.
Zygmunt, B. and Namiesnik, J. (2003) Preparation of samples of plant material for chromatographic analysis. J. Chromatogr. Sci. 41, 109–116.
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Seidel, V. (2006). Initial and Bulk Extraction. In: Sarker, S.D., Latif, Z., Gray, A.I. (eds) Natural Products Isolation. Methods in Biotechnology, vol 20. Humana Press. https://doi.org/10.1385/1-59259-955-9:27
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DOI: https://doi.org/10.1385/1-59259-955-9:27
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Print ISBN: 978-1-58829-447-0
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