Abstract
Antibodies reacting to specific cell surface markers can be bound to magnetic beads and used to specifically capture cells exhibiting the marker. This approach allows the selective enrichment of specific cell subpopulations and as the methods are amenable to use in blood, tissue fluids and culture medium living cells can be recovered that can subsequently be subcultured. Negative cell separation can also be used where undesirable cell types are tagged using magnetic beads and removed from the desirable cell population.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Levine, S. (1856) Magnetic techniques for in vitro isolation of leucocytes, Science 123, 185.
Melville, D., Paul, F., and Roath, S. (1975) High gradient magnetic separation of red cells from whole blood. IEEE Trans. Magn. 1701.
Uhlen, M., Hornes, E., and Olsvik, O. (1994) Advances in Biomagnetic Separation, Eaton Publishing Co., Natick, MA
Gee, A. P. (1998) Immunomagnetic cell separation using antibodies and superparamagnetic microspheres, in Cell Separation Methods and Applications (Recktenwald, D., and Radbruch, A., eds.), Marcel Dekker, Inc., New York, pp. 175.
Zborowski, M. (1997) Physics of magnetic cell sorting, in Scientific and Clinical Applications of Magnetic Carriers (Haefeli, U., Schuett, W., Teller, J., and Zborowski, M., eds.), Plenum Press, New York, pp. 205.
Hatch, G. P. and Stelter, R. E. (2001) Magnetic design considerations for devices and particles used for biological high-gradient magnetic separation (HGMS) systems. J. Magnetism Magn. Mater. 225, 262.
Coey, M. and Weaire, D. (1998) Magnets, markets, and magic cylinders. The Industrial Physicist September, 34.
Ugelstad, J., Stenstad, P., Kilaas, L., Prestvik, W. S., Herje, R., Berge, A., et al. (1993) Monodisperse magnetic polymer particles. New biochemical and biomedical applications. Blood Purif. 11, 34.
Kantor, A. B., Gibbons, I., Miltenyi, S., and Schmitz, J. (1998) Magnetic cell sorting with colloidal superparamagnetic particles, in Cell Separation Methods and Applications (Recktenwald, D., and Radbruch, A., eds.), Marcel Dekker, Inc., New York, pp. 153.
Liberti, P. A., Rao, C. G., and Terstappen, L. W. M. M. (2001) Optimization of ferrofluids and protocols for the enrichment of breast tumor cells in blood. J. Magnetism Magnetic Mater. 301, 301.
Thomas, T. E., Richards, A. J., Roath, O. S., Watson, J. H. P., Smith, R. J. S., and Lansdorp, P. M. (1993) Positive selection of human blood cells using improved high gradient magnetic separation filters. J. Hematother. 2, 297.
Dyer, P. A., Brown, P., and Edward, R. (1998) Immunomethods: magnetic, column and panning techniques, in Cell Separation: A Practical Approach (Fisher, D., Francis, G. E., and Rickwood, D., eds.), Oxford University Press, Oxford, p. 191.
Treleaven, J. G., Gibson, F. M., Ugelstad, J., Rembaum, A., Philip, T., Caine, G. D., and Kemshead, J. T. (1984) Removal of neuroblastoma cells from bone marrow with monoclonal antibodies conjugated to magnetic microspheres, Lancet 1, 70.
Pole, J. G., Gee, A., Janssen, W., Lee, C., and Gross, S. (1990) Immunomagnetic purging of bone marrow: a model for negative cell selection. Am. J. Pediatr. Hematology/Oncol. 12, 257.
Shapiro, H. M. (1995) Practical Flow Cytometry, Wiley-Liss, Inc., New York.
Miltenyi, S., Müller, W., Weichel, W., and Radbruch, A. (1990) High gradient magnetic cell separation with MACS. Cytometry 11, 231.
Comella, K., Nakamura, M., Melnik, K., Chosy, J., Zborowski, M., Cooper, M. A., et al. (2001) Effects of antibody concentration on the separation of human natural killer cells in a commercial immunomagnetic separation system. Cytometry 45, 285.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Zborowski, M., Chalmers, J.J. (2005). Magnetic Cell Sorting. In: Burns, R. (eds) Immunochemical Protocols. Methods In Molecular Biology™, vol 295. Humana Press. https://doi.org/10.1385/1-59259-873-0:291
Download citation
DOI: https://doi.org/10.1385/1-59259-873-0:291
Publisher Name: Humana Press
Print ISBN: 978-1-58829-274-2
Online ISBN: 978-1-59259-873-1
eBook Packages: Springer Protocols