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Genetic Transformation of Mature Citrus Plants

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Transgenic Plants: Methods and Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 286))

Summary

Most woody fruit species have long juvenile periods that drastically prolong the time required to analyze mature traits. Evaluation of characteristics related to fruits is a requisite to release any new variety into the market. Because of a decline in regenerative and transformation potential, genetic transformation procedures usually employ juvenile material as the source of plant tissue, therefore resulting in the production of juvenile plants. Direct transformation of mature material could ensure the production of adult transgenic plants, bypassing in this way the juvenile phase. Invigoration of the source adult material, establishment of adequate transformation and regeneration conditions, and acceleration of plant development through grafting allowed us to produce transgenic mature sweet orange trees flowering and bearing fruits in a short time period.

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Author information

Authors and Affiliations

  1. Department of Plant Protection and Biotechnology, Instituto Valenciano de Investigaciones Agrarias, Valencia, Spain

    Magdalena Cervera, José Juárez, Luis Navarro & Leandro Peña

Authors
  1. Magdalena Cervera
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  2. José Juárez
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  3. Luis Navarro
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  4. Leandro Peña
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Editor information

Editors and Affiliations

  1. Instituto Valenciano de Investigaciones Agrarias, Valencia, Spain

    Leandro Peña

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© 2005 Humana Press Inc.

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Cervera, M., Juárez, J., Navarro, L., Peña, L. (2005). Genetic Transformation of Mature Citrus Plants. In: Peña, L. (eds) Transgenic Plants: Methods and Protocols. Methods in Molecular Biology™, vol 286. Humana Press. https://doi.org/10.1385/1-59259-827-7:177

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  • DOI: https://doi.org/10.1385/1-59259-827-7:177

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-263-6

  • Online ISBN: 978-1-59259-827-4

  • eBook Packages: Springer Protocols

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